Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells
2012
Authors
Najman, StevoStojanović, Sanja
Mitrić, Žarko
Ajduković, Zorica
Ignjatović, Nenad L.
Uskoković, Dragan
Contributors
Grbović-Novaković, JasminaIgnjatović, Nenad L.
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We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.
Keywords:
calcium phosphate / poly(DL-lactide-co-glycolide) / Saos2 cells / cobalt-exchanged hydroxyapatite / biomaterialsSource:
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts, 2012, 110-110Publisher:
- Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
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VinčaTY - CONF AU - Najman, Stevo AU - Stojanović, Sanja AU - Mitrić, Žarko AU - Ajduković, Zorica AU - Ignjatović, Nenad L. AU - Uskoković, Dragan PY - 2012 UR - http://dais.sanu.ac.rs/123456789/519 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7466 AB - We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity. PB - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade C3 - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts T1 - Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells SP - 110 EP - 110 UR - https://hdl.handle.net/21.15107/rcub_vinar_7466 ER -
@conference{ author = "Najman, Stevo and Stojanović, Sanja and Mitrić, Žarko and Ajduković, Zorica and Ignjatović, Nenad L. and Uskoković, Dragan", year = "2012", abstract = "We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.", publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade", journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts", title = "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells", pages = "110-110", url = "https://hdl.handle.net/21.15107/rcub_vinar_7466" }
Najman, S., Stojanović, S., Mitrić, Ž., Ajduković, Z., Ignjatović, N. L.,& Uskoković, D.. (2012). Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 110-110. https://hdl.handle.net/21.15107/rcub_vinar_7466
Najman S, Stojanović S, Mitrić Ž, Ajduković Z, Ignjatović NL, Uskoković D. Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:110-110. https://hdl.handle.net/21.15107/rcub_vinar_7466 .
Najman, Stevo, Stojanović, Sanja, Mitrić, Žarko, Ajduković, Zorica, Ignjatović, Nenad L., Uskoković, Dragan, "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells" in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):110-110, https://hdl.handle.net/21.15107/rcub_vinar_7466 .