Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine
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The proposed method is based on coloured hydrazone formation with 1,4-dihydrazinophthalazine as a reagent. Heating at 85 degrees C for 2 h was found necessary to ensure optimal hydrazone formation in the presence of hydrochloric acid. The yellow hydrazone product has an absorption maximum at 380 nm. A linear relationship between absorbance and concentration was established in the concentration range 3.19 x 10(-6)-3.19 x 10(-5) mol l(-1) (the regression equation was y = 0.013 167 3 + 0.019 025 9x; correlation coefficient r = 0.9991; n = 6). The detection limit was 1.2 mu g ml(-1) (molar absorptivity found was 1.97 x 10(4) l mol(-1) cm(-1)). The reliability of the proposed method was checked at three different concentrations; the relative standard deviation (RSD) varied from 1.03 to 2.01%. The described method applied to the determination of desoximetasone in ointment gave precise and reproducible results; the recovery was 98.55% with RSD = 2.40% (n = 10).
Keywords:desoximetasone / 1,4-dihydrazinophthalazine / ointment / spectrophotometry
Source:Journal of Pharmaceutical and Biomedical Analysis, 1996, 14, 8-10, 947-950
- 5th International Symposium on Drug Analysis, Sep 12-15, 1995, Louvain, Belgium