Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome
Само за регистроване кориснике
2015
Аутори
Tepavčević, SnežanaMilutinovic, Danijela Vojnovic
Macut, Djuro
Stojiljković, Mojca D.
Nikolić, Marina
Bozic-Antic, Ivana
Ćulafić, Tijana
Bjekic-Macut, Jelica
Matić, Gordana
Korićanac, Goran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, ...total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.
Кључне речи:
Polycystic ovary syndrome / Heart / Fatty acid transport / Fatty acid oxidation / Cardiac triglyceridesИзвор:
Endocrine, 2015, 50, 1, 193-201Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.1007/s12020-015-0558-1
ISSN: 1355-008X; 1559-0100
PubMed: 25702158
WoS: 000360192500026
Scopus: 2-s2.0-84940438855
Колекције
Институција/група
VinčaTY - JOUR AU - Tepavčević, Snežana AU - Milutinovic, Danijela Vojnovic AU - Macut, Djuro AU - Stojiljković, Mojca D. AU - Nikolić, Marina AU - Bozic-Antic, Ivana AU - Ćulafić, Tijana AU - Bjekic-Macut, Jelica AU - Matić, Gordana AU - Korićanac, Goran PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/710 AB - Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation. T2 - Endocrine T1 - Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome VL - 50 IS - 1 SP - 193 EP - 201 DO - 10.1007/s12020-015-0558-1 ER -
@article{ author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Stojiljković, Mojca D. and Nikolić, Marina and Bozic-Antic, Ivana and Ćulafić, Tijana and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran", year = "2015", abstract = "Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.", journal = "Endocrine", title = "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome", volume = "50", number = "1", pages = "193-201", doi = "10.1007/s12020-015-0558-1" }
Tepavčević, S., Milutinovic, D. V., Macut, D., Stojiljković, M. D., Nikolić, M., Bozic-Antic, I., Ćulafić, T., Bjekic-Macut, J., Matić, G.,& Korićanac, G.. (2015). Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. in Endocrine, 50(1), 193-201. https://doi.org/10.1007/s12020-015-0558-1
Tepavčević S, Milutinovic DV, Macut D, Stojiljković MD, Nikolić M, Bozic-Antic I, Ćulafić T, Bjekic-Macut J, Matić G, Korićanac G. Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. in Endocrine. 2015;50(1):193-201. doi:10.1007/s12020-015-0558-1 .
Tepavčević, Snežana, Milutinovic, Danijela Vojnovic, Macut, Djuro, Stojiljković, Mojca D., Nikolić, Marina, Bozic-Antic, Ivana, Ćulafić, Tijana, Bjekic-Macut, Jelica, Matić, Gordana, Korićanac, Goran, "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome" in Endocrine, 50, no. 1 (2015):193-201, https://doi.org/10.1007/s12020-015-0558-1 . .