Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media
Само за регистроване кориснике
2015
Аутори
Jotić, AnaJešić, Snežana
Živković, Maja
Tomanović, Nada
Kuveljić, Jovana
Stanković, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective: Toll-like receptors (TLRs) have a prominent role in inducing innate immune response. It has been suggested that regulation of TLRs is involved in the pathogenesis of chronic otitis media. TLR 2 and TLR 4 polymorphisms were connected with susceptibility to acute otitis and chronic otitis with effusion. The objective of this study was to establish expression of TLR 2 and 4 on middle ear mucosa in different types of chronic suppurative otitis media (CSOM), and the influence of gene polymorphisms TLR 2 Arg753Gln and TLR 4 Thr399Ile and Asp299Gly to susceptibility to CSOM. Material and methods: Middle ear mucosa and full blood samples were obtained from 85 patients With chronic suppurative otitis media with and without cholesteatoma. Control group for mucosal TLR expression consisted of 71 samples of middle ear mucosa taken from patients with otosclerosis, and control group for DNA polymorphism consisted of 100 full blood samples in healthy subjects. DNA polymorphism detection wa...s done with restriction fragment length polymorphism in RI PCR. Expression of TLR 2 and 4 was determined with immunohistochemical staining. Results: TLR 2 and TLR 4 expression on the middle ear mucosa was not influenced by age of the patients with chronic otitis media. Incidence of TLR 2 Arg753Gln polymorphism was significantly higher in patients with chronic otitis media, compared to control group. Significant association between TLR 2 Arg753Gln polymorphism and different types of mucosal changes in patients with chronic otitis media was established. TLR 2 and 4 expression on experimental group mucosa was significantly different compared to control group, where there was no expression (p = 0.000). Strong dependence of TLR 2 and TLR 4 expression on middle ear mucosa with different mucosal changes and immunohistochemical activity after staining was detected. Conclusion: Certain polymorphisms in TLR genes could be indicative for susceptibility to chronic otitis media. Expression of TLR 2 and 4 on middle ear mucosa was more dependable on different types of mucosal changes and type of CSOM than on bacteria found in the specimens. This can indicate that the type of mucosal changes are closely correlated with TLRs activity in middle ear. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Кључне речи:
Chronic otitis media / Toll-like receptor (TLR) 2 / TLR 4Извор:
Auris Nasus Larynx, 2015, 42, 6, 431-437Финансирање / пројекти:
DOI: 10.1016/j.anl.2015.04.010
ISSN: 0385-8146; 1879-1476
PubMed: 26055429
WoS: 000358632000001
Scopus: 2-s2.0-84937524677
Колекције
Институција/група
VinčaTY - JOUR AU - Jotić, Ana AU - Ješić, Snežana AU - Živković, Maja AU - Tomanović, Nada AU - Kuveljić, Jovana AU - Stanković, Aleksandra PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/679 AB - Objective: Toll-like receptors (TLRs) have a prominent role in inducing innate immune response. It has been suggested that regulation of TLRs is involved in the pathogenesis of chronic otitis media. TLR 2 and TLR 4 polymorphisms were connected with susceptibility to acute otitis and chronic otitis with effusion. The objective of this study was to establish expression of TLR 2 and 4 on middle ear mucosa in different types of chronic suppurative otitis media (CSOM), and the influence of gene polymorphisms TLR 2 Arg753Gln and TLR 4 Thr399Ile and Asp299Gly to susceptibility to CSOM. Material and methods: Middle ear mucosa and full blood samples were obtained from 85 patients With chronic suppurative otitis media with and without cholesteatoma. Control group for mucosal TLR expression consisted of 71 samples of middle ear mucosa taken from patients with otosclerosis, and control group for DNA polymorphism consisted of 100 full blood samples in healthy subjects. DNA polymorphism detection was done with restriction fragment length polymorphism in RI PCR. Expression of TLR 2 and 4 was determined with immunohistochemical staining. Results: TLR 2 and TLR 4 expression on the middle ear mucosa was not influenced by age of the patients with chronic otitis media. Incidence of TLR 2 Arg753Gln polymorphism was significantly higher in patients with chronic otitis media, compared to control group. Significant association between TLR 2 Arg753Gln polymorphism and different types of mucosal changes in patients with chronic otitis media was established. TLR 2 and 4 expression on experimental group mucosa was significantly different compared to control group, where there was no expression (p = 0.000). Strong dependence of TLR 2 and TLR 4 expression on middle ear mucosa with different mucosal changes and immunohistochemical activity after staining was detected. Conclusion: Certain polymorphisms in TLR genes could be indicative for susceptibility to chronic otitis media. Expression of TLR 2 and 4 on middle ear mucosa was more dependable on different types of mucosal changes and type of CSOM than on bacteria found in the specimens. This can indicate that the type of mucosal changes are closely correlated with TLRs activity in middle ear. (C) 2015 Elsevier Ireland Ltd. All rights reserved. T2 - Auris Nasus Larynx T1 - Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media VL - 42 IS - 6 SP - 431 EP - 437 DO - 10.1016/j.anl.2015.04.010 ER -
@article{ author = "Jotić, Ana and Ješić, Snežana and Živković, Maja and Tomanović, Nada and Kuveljić, Jovana and Stanković, Aleksandra", year = "2015", abstract = "Objective: Toll-like receptors (TLRs) have a prominent role in inducing innate immune response. It has been suggested that regulation of TLRs is involved in the pathogenesis of chronic otitis media. TLR 2 and TLR 4 polymorphisms were connected with susceptibility to acute otitis and chronic otitis with effusion. The objective of this study was to establish expression of TLR 2 and 4 on middle ear mucosa in different types of chronic suppurative otitis media (CSOM), and the influence of gene polymorphisms TLR 2 Arg753Gln and TLR 4 Thr399Ile and Asp299Gly to susceptibility to CSOM. Material and methods: Middle ear mucosa and full blood samples were obtained from 85 patients With chronic suppurative otitis media with and without cholesteatoma. Control group for mucosal TLR expression consisted of 71 samples of middle ear mucosa taken from patients with otosclerosis, and control group for DNA polymorphism consisted of 100 full blood samples in healthy subjects. DNA polymorphism detection was done with restriction fragment length polymorphism in RI PCR. Expression of TLR 2 and 4 was determined with immunohistochemical staining. Results: TLR 2 and TLR 4 expression on the middle ear mucosa was not influenced by age of the patients with chronic otitis media. Incidence of TLR 2 Arg753Gln polymorphism was significantly higher in patients with chronic otitis media, compared to control group. Significant association between TLR 2 Arg753Gln polymorphism and different types of mucosal changes in patients with chronic otitis media was established. TLR 2 and 4 expression on experimental group mucosa was significantly different compared to control group, where there was no expression (p = 0.000). Strong dependence of TLR 2 and TLR 4 expression on middle ear mucosa with different mucosal changes and immunohistochemical activity after staining was detected. Conclusion: Certain polymorphisms in TLR genes could be indicative for susceptibility to chronic otitis media. Expression of TLR 2 and 4 on middle ear mucosa was more dependable on different types of mucosal changes and type of CSOM than on bacteria found in the specimens. This can indicate that the type of mucosal changes are closely correlated with TLRs activity in middle ear. (C) 2015 Elsevier Ireland Ltd. All rights reserved.", journal = "Auris Nasus Larynx", title = "Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media", volume = "42", number = "6", pages = "431-437", doi = "10.1016/j.anl.2015.04.010" }
Jotić, A., Ješić, S., Živković, M., Tomanović, N., Kuveljić, J.,& Stanković, A.. (2015). Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media. in Auris Nasus Larynx, 42(6), 431-437. https://doi.org/10.1016/j.anl.2015.04.010
Jotić A, Ješić S, Živković M, Tomanović N, Kuveljić J, Stanković A. Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media. in Auris Nasus Larynx. 2015;42(6):431-437. doi:10.1016/j.anl.2015.04.010 .
Jotić, Ana, Ješić, Snežana, Živković, Maja, Tomanović, Nada, Kuveljić, Jovana, Stanković, Aleksandra, "Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media" in Auris Nasus Larynx, 42, no. 6 (2015):431-437, https://doi.org/10.1016/j.anl.2015.04.010 . .