Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities
Апстракт
The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an ordinary quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated ...hydrogen (H-center dot) atom. (C) 2007 Elsevier B.V. All rights reserved.
Кључне речи:
EPR spectroscopy / spin-traps / DEPMPO / Fenton reaction / hydrogen radicalИзвор:
Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy, 2008, 69, 5, 1354-1366Напомена:
- 10th International Workshop on Electron Magnetic Resonance of Disordered Systems, Jun 07-14, 2007, Sophia, Bulgaria
DOI: 10.1016/j.saa.2007.09.047
ISSN: 1386-1425
PubMed: 18255335
WoS: 000256180800011
Scopus: 2-s2.0-42149158251
Колекције
Институција/група
VinčaTY - JOUR AU - Bačić, Goran AU - Spasojević, Ivan AU - Šećerov, Bojana Lj. AU - Mojovic, Milos PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6755 AB - The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an ordinary quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom. (C) 2007 Elsevier B.V. All rights reserved. T2 - Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy T1 - Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities VL - 69 IS - 5 SP - 1354 EP - 1366 DO - 10.1016/j.saa.2007.09.047 ER -
@article{ author = "Bačić, Goran and Spasojević, Ivan and Šećerov, Bojana Lj. and Mojovic, Milos", year = "2008", abstract = "The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an ordinary quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom. (C) 2007 Elsevier B.V. All rights reserved.", journal = "Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy", title = "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities", volume = "69", number = "5", pages = "1354-1366", doi = "10.1016/j.saa.2007.09.047" }
Bačić, G., Spasojević, I., Šećerov, B. Lj.,& Mojovic, M.. (2008). Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy, 69(5), 1354-1366. https://doi.org/10.1016/j.saa.2007.09.047
Bačić G, Spasojević I, Šećerov BL, Mojovic M. Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy. 2008;69(5):1354-1366. doi:10.1016/j.saa.2007.09.047 .
Bačić, Goran, Spasojević, Ivan, Šećerov, Bojana Lj., Mojovic, Milos, "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities" in Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy, 69, no. 5 (2008):1354-1366, https://doi.org/10.1016/j.saa.2007.09.047 . .