Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation
Нема приказа
Аутори
Petrović, Ivan M.Korićanac, Lela
Todorović, Danijela V.
Ristić-Fira, Aleksandra
Valastro, Lucia M.
Privitera, Giuseppe
Cuttone, Giacomo
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more re...sistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs.
Кључне речи:
hTB140 melanoma cells / fotemustine / dacarbazine / proton irradiation / viability / cell survivalИзвор:
Annals of the New York Academy of Sciences, 2007, 1095, 154-164Напомена:
- Cell Signaling World 2006 Conference, Jan 25-28, 2006, Luxembourg, Luxembourg
DOI: 10.1196/annals.1397.019
ISSN: 0077-8923
PubMed: 17404028
WoS: 000245947400018
Scopus: 2-s2.0-34247844384
Колекције
Институција/група
VinčaTY - JOUR AU - Petrović, Ivan M. AU - Korićanac, Lela AU - Todorović, Danijela V. AU - Ristić-Fira, Aleksandra AU - Valastro, Lucia M. AU - Privitera, Giuseppe AU - Cuttone, Giacomo PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6647 AB - Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more resistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs. T2 - Annals of the New York Academy of Sciences T1 - Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation VL - 1095 SP - 154 EP - 164 DO - 10.1196/annals.1397.019 ER -
@article{ author = "Petrović, Ivan M. and Korićanac, Lela and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Valastro, Lucia M. and Privitera, Giuseppe and Cuttone, Giacomo", year = "2007", abstract = "Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more resistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs.", journal = "Annals of the New York Academy of Sciences", title = "Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation", volume = "1095", pages = "154-164", doi = "10.1196/annals.1397.019" }
Petrović, I. M., Korićanac, L., Todorović, D. V., Ristić-Fira, A., Valastro, L. M., Privitera, G.,& Cuttone, G.. (2007). Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation. in Annals of the New York Academy of Sciences, 1095, 154-164. https://doi.org/10.1196/annals.1397.019
Petrović IM, Korićanac L, Todorović DV, Ristić-Fira A, Valastro LM, Privitera G, Cuttone G. Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation. in Annals of the New York Academy of Sciences. 2007;1095:154-164. doi:10.1196/annals.1397.019 .
Petrović, Ivan M., Korićanac, Lela, Todorović, Danijela V., Ristić-Fira, Aleksandra, Valastro, Lucia M., Privitera, Giuseppe, Cuttone, Giacomo, "Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation" in Annals of the New York Academy of Sciences, 1095 (2007):154-164, https://doi.org/10.1196/annals.1397.019 . .