Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy
Apstrakt
Front-face synchronous fluorescence spectroscopy combined with chemometrics is used to classify honey samples according to their botanical origin. Synchronous fluorescence spectra of three monofloral (linden, sunflower, and acacia), polyfloral (meadow mix), and fake (fake acacia and linden) honey types (109 samples) were collected in an excitation range of 240-500 nm for synchronous wavelength intervals of 30-300 nm. Chemometric analysis of the gathered data included principal component analysis and partial least squares discriminant analysis. Mean cross-validated classification errors of 0.2 and 4.8% were found for a model that accounts only for monofloral samples and for a model that includes both the monofloral and polyfloral groups, respectively. The results demonstrate that single synchronous fluorescence spectra of different honeys differ significantly because of their distinct physical and chemical characteristics and provide sufficient data for the clear differentiation among h...oney groups. The spectra of fake honey samples showed pronounced differences from those of genuine honey, and these samples are easily recognized on the basis of their synchronous fluorescence spectra. The study demonstrated that this method is a valuable and promising technique for honey authentication.
Ključne reči:
Honey / Botanical origin / Fluorescence spectroscopy / Food authentication / ChemometricsIzvor:
Applied Spectroscopy, 2014, 68, 5, 557-563Finansiranje / projekti:
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
DOI: 10.1366/13-07325
ISSN: 0003-7028; 1943-3530
PubMed: 25014599
WoS: 000335295400007
Scopus: 2-s2.0-84911967659
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Lenhardt, Lea I. AU - Zeković, Ivana Lj. AU - Dramićanin, Tatjana AU - Dramićanin, Miroslav AU - Bro, Rasmus PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5981 AB - Front-face synchronous fluorescence spectroscopy combined with chemometrics is used to classify honey samples according to their botanical origin. Synchronous fluorescence spectra of three monofloral (linden, sunflower, and acacia), polyfloral (meadow mix), and fake (fake acacia and linden) honey types (109 samples) were collected in an excitation range of 240-500 nm for synchronous wavelength intervals of 30-300 nm. Chemometric analysis of the gathered data included principal component analysis and partial least squares discriminant analysis. Mean cross-validated classification errors of 0.2 and 4.8% were found for a model that accounts only for monofloral samples and for a model that includes both the monofloral and polyfloral groups, respectively. The results demonstrate that single synchronous fluorescence spectra of different honeys differ significantly because of their distinct physical and chemical characteristics and provide sufficient data for the clear differentiation among honey groups. The spectra of fake honey samples showed pronounced differences from those of genuine honey, and these samples are easily recognized on the basis of their synchronous fluorescence spectra. The study demonstrated that this method is a valuable and promising technique for honey authentication. T2 - Applied Spectroscopy T1 - Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy VL - 68 IS - 5 SP - 557 EP - 563 DO - 10.1366/13-07325 ER -
@article{ author = "Lenhardt, Lea I. and Zeković, Ivana Lj. and Dramićanin, Tatjana and Dramićanin, Miroslav and Bro, Rasmus", year = "2014", abstract = "Front-face synchronous fluorescence spectroscopy combined with chemometrics is used to classify honey samples according to their botanical origin. Synchronous fluorescence spectra of three monofloral (linden, sunflower, and acacia), polyfloral (meadow mix), and fake (fake acacia and linden) honey types (109 samples) were collected in an excitation range of 240-500 nm for synchronous wavelength intervals of 30-300 nm. Chemometric analysis of the gathered data included principal component analysis and partial least squares discriminant analysis. Mean cross-validated classification errors of 0.2 and 4.8% were found for a model that accounts only for monofloral samples and for a model that includes both the monofloral and polyfloral groups, respectively. The results demonstrate that single synchronous fluorescence spectra of different honeys differ significantly because of their distinct physical and chemical characteristics and provide sufficient data for the clear differentiation among honey groups. The spectra of fake honey samples showed pronounced differences from those of genuine honey, and these samples are easily recognized on the basis of their synchronous fluorescence spectra. The study demonstrated that this method is a valuable and promising technique for honey authentication.", journal = "Applied Spectroscopy", title = "Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy", volume = "68", number = "5", pages = "557-563", doi = "10.1366/13-07325" }
Lenhardt, L. I., Zeković, I. Lj., Dramićanin, T., Dramićanin, M.,& Bro, R.. (2014). Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy. in Applied Spectroscopy, 68(5), 557-563. https://doi.org/10.1366/13-07325
Lenhardt LI, Zeković IL, Dramićanin T, Dramićanin M, Bro R. Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy. in Applied Spectroscopy. 2014;68(5):557-563. doi:10.1366/13-07325 .
Lenhardt, Lea I., Zeković, Ivana Lj., Dramićanin, Tatjana, Dramićanin, Miroslav, Bro, Rasmus, "Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy" in Applied Spectroscopy, 68, no. 5 (2014):557-563, https://doi.org/10.1366/13-07325 . .