Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome
Нема приказа
Аутори
Tepavčević, SnežanaMilutinovic, Danijela Vojnovic
Macut, Djuro
Žakula, Zorica
Nikolić, Marina
Bozic-Antic, Ivana
Romić, Snježana Đ.
Bjekic-Macut, Jelica
Matić, Gordana
Korićanac, Goran
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was in...creased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.
Кључне речи:
Polycystic ovary syndrome / Dihydrotestosterone / Heart / Insulin signaling pathway / Glucose transportersИзвор:
Journal of Steroid Biochemistry and Molecular Biology, 2014, 141, 71-76Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.1016/j.jsbmb.2014.01.006
ISSN: 0960-0760
PubMed: 24472754
WoS: 000335114500009
Scopus: 2-s2.0-84893964210
Колекције
Институција/група
VinčaTY - JOUR AU - Tepavčević, Snežana AU - Milutinovic, Danijela Vojnovic AU - Macut, Djuro AU - Žakula, Zorica AU - Nikolić, Marina AU - Bozic-Antic, Ivana AU - Romić, Snježana Đ. AU - Bjekic-Macut, Jelica AU - Matić, Gordana AU - Korićanac, Goran PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5970 AB - It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved. T2 - Journal of Steroid Biochemistry and Molecular Biology T1 - Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome VL - 141 SP - 71 EP - 76 DO - 10.1016/j.jsbmb.2014.01.006 ER -
@article{ author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Žakula, Zorica and Nikolić, Marina and Bozic-Antic, Ivana and Romić, Snježana Đ. and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran", year = "2014", abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.", journal = "Journal of Steroid Biochemistry and Molecular Biology", title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome", volume = "141", pages = "71-76", doi = "10.1016/j.jsbmb.2014.01.006" }
Tepavčević, S., Milutinovic, D. V., Macut, D., Žakula, Z., Nikolić, M., Bozic-Antic, I., Romić, S. Đ., Bjekic-Macut, J., Matić, G.,& Korićanac, G.. (2014). Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology, 141, 71-76. https://doi.org/10.1016/j.jsbmb.2014.01.006
Tepavčević S, Milutinovic DV, Macut D, Žakula Z, Nikolić M, Bozic-Antic I, Romić SĐ, Bjekic-Macut J, Matić G, Korićanac G. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology. 2014;141:71-76. doi:10.1016/j.jsbmb.2014.01.006 .
Tepavčević, Snežana, Milutinovic, Danijela Vojnovic, Macut, Djuro, Žakula, Zorica, Nikolić, Marina, Bozic-Antic, Ivana, Romić, Snježana Đ., Bjekic-Macut, Jelica, Matić, Gordana, Korićanac, Goran, "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome" in Journal of Steroid Biochemistry and Molecular Biology, 141 (2014):71-76, https://doi.org/10.1016/j.jsbmb.2014.01.006 . .