Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol
AuthorsBundalo, Maja M.
MetadataShow full item record
The renin-angiotensin system has been implicated in the development of metabolic syndrome and appears to be a key in the local tissue control of normal cardiac functions. Physiological concentrations of estrogens have been shown to be cardioprotective, especially against the damaging effects of fructose-rich diet. The aim of the study was to investigate the expression of the renin-angiotensin system molecules with potentially deleterious effect on the heart (angiotensin-converting enzyme and angiotensin II type 1 receptor) and those with potentially protective effects, (angiotensin-converting enzyme 2 and angiotensin II type 2 receptor), in ovariectomized fructose fed female rats with 17-estradiol replacement. Real-time PCR and Western blot analysis were used for quantification of gene and protein expression in the heart. Fructose diet increased the expression of angiotensin-converting enzyme and angiotensin II type 1 receptor and decreased the expression of angiotensin-converting enzy...me 2 and angiotensin II type 2 receptor. On the other hand, estradiol replacement seems to undo fructose diet effects on cardiac renin-angiotensin system. Downregulation of angiotensin-converting enzyme and angiotensin II type 1 receptor, and reversion of expression of both potentially protective molecules, angiotensin-converting enzyme 2 and angiotensin II type 2 receptor, to the control level in cardiac tissue took place. Obtained results suggest that estradiol may reverse the harmful effect of fructose-rich diet on the expression of renin-angiotensin system molecules. These findings may also be important in further research of phenotypes like insulin resistance, metabolic syndrome, and following cardiovascular pathology in females.
Keywords:metabolic syndrome / ovariectomy / angiotensin converting enzyme / angiotensin converting enzyme 2 / angiotensin II type 1 receptor / angiotensin II type 2 receptor
Source:Hormone and Metabolic Research, 2015, 47, 7, 521-527
Showing items related by title, author, creator and subject.
The effect of DNA polymorphisms in angiotensin I-converting enzyme and angiotensin II type1 receptor genes with arterial blood pressure levels in Serbian population Stanković, Aleksandra; Živković, Maja; Đurić, Tamara; Alavantić, Dragan (European Journal of Human Genetics, 2002)
Gene polymorphisms of the angiotensin I-converting enzyme and angiotensin II type 1 receptor A1166C in non-insulin dependent diabetes mellitus Živković, Maja; Stanković, Aleksandra; Sumarac-Dumanovic, M; Glišić, Sanja (I); Micic, D; Alavantić, Dragan (European Journal of Human Genetics, 2002)
Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta Bundalo, Maja M.; Živković, Maja; Romić, Snježana Đ.; Tepavčević, Snežana; Korićanac, Goran; Đurić, Tamara; Stanković, Aleksandra (Journal of the Renin-Angiotensin-Aldosterone System, 2016)