Maprotiline treatment differentially influences cardiac beta-adrenoreceptors expression under normal and stress conditions
Апстракт
Alterations in cardiac function were observed in antidepressants treated patients and published in several clinical reports. These detected changes could be either a consequence of the treatment or of depression itself, which has already been proved to be a risk factor in heart diseases. In order to determine a possible influence of chronic treatment with norepinephrinergic reuptake inhibitor, maprotiline, on the heart, we investigated gene expression of cardiac beta-adrenoceptors both in controls and in animals with signs of depression. The rats were divided into two groups, unstressed controls and those exposed to chronic unpredictable mild stress (CUMS). The groups were further divided into two subgroups, one receiving daily intraperitoneal injections of vehicle (sterile water) and another one maprotiline (10 mg/kg) for four weeks. Tissue samples were collected after the last application. Gene expression of cardiac beta(1)- and beta(2)-adrenoceptor was determined using Real-time RT-...PCR analysis. Our results show that in control animals expression of both adrenoreceptors was decreased in the right atria after 4 weeks of maprotiline application. Contrary, the same treatment led to a significant increase in expression of cardiac beta(1)-adrenoceptor in the stressed rats, with no change in the characteristics of beta(2)-adrenoceptor. Our findings might reflect the that molecular mechanisms are underlying factors involved in the development of cardiovascular diseases linked with antidepressant treatment.
Кључне речи:
Antidepressants / Depression/treatment / Cardiovascular diseases/associated with the use of antidepressants / beta-adrenoceptors/gene expressionИзвор:
Brazilian Journal of Pharmaceutical Sciences, 2012, 48, 4, 793-799Финансирање / пројекти:
- Молекуларни механизми патофизиолошких промена у ћелијама централног нервног система и периферног ткива код сисара (RS-MESTD-Basic Research (BR or ON)-173044)
DOI: 10.1590/S1984-82502012000400023
ISSN: 1984-8250
WoS: 000324585500024
Scopus: 2-s2.0-84874225525
Колекције
Институција/група
VinčaTY - JOUR AU - Spasojević, Nataša AU - Jovanović, Predrag AU - Dronjak, Slađana PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5693 AB - Alterations in cardiac function were observed in antidepressants treated patients and published in several clinical reports. These detected changes could be either a consequence of the treatment or of depression itself, which has already been proved to be a risk factor in heart diseases. In order to determine a possible influence of chronic treatment with norepinephrinergic reuptake inhibitor, maprotiline, on the heart, we investigated gene expression of cardiac beta-adrenoceptors both in controls and in animals with signs of depression. The rats were divided into two groups, unstressed controls and those exposed to chronic unpredictable mild stress (CUMS). The groups were further divided into two subgroups, one receiving daily intraperitoneal injections of vehicle (sterile water) and another one maprotiline (10 mg/kg) for four weeks. Tissue samples were collected after the last application. Gene expression of cardiac beta(1)- and beta(2)-adrenoceptor was determined using Real-time RT-PCR analysis. Our results show that in control animals expression of both adrenoreceptors was decreased in the right atria after 4 weeks of maprotiline application. Contrary, the same treatment led to a significant increase in expression of cardiac beta(1)-adrenoceptor in the stressed rats, with no change in the characteristics of beta(2)-adrenoceptor. Our findings might reflect the that molecular mechanisms are underlying factors involved in the development of cardiovascular diseases linked with antidepressant treatment. T2 - Brazilian Journal of Pharmaceutical Sciences T1 - Maprotiline treatment differentially influences cardiac beta-adrenoreceptors expression under normal and stress conditions VL - 48 IS - 4 SP - 793 EP - 799 DO - 10.1590/S1984-82502012000400023 ER -
@article{ author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana", year = "2012", abstract = "Alterations in cardiac function were observed in antidepressants treated patients and published in several clinical reports. These detected changes could be either a consequence of the treatment or of depression itself, which has already been proved to be a risk factor in heart diseases. In order to determine a possible influence of chronic treatment with norepinephrinergic reuptake inhibitor, maprotiline, on the heart, we investigated gene expression of cardiac beta-adrenoceptors both in controls and in animals with signs of depression. The rats were divided into two groups, unstressed controls and those exposed to chronic unpredictable mild stress (CUMS). The groups were further divided into two subgroups, one receiving daily intraperitoneal injections of vehicle (sterile water) and another one maprotiline (10 mg/kg) for four weeks. Tissue samples were collected after the last application. Gene expression of cardiac beta(1)- and beta(2)-adrenoceptor was determined using Real-time RT-PCR analysis. Our results show that in control animals expression of both adrenoreceptors was decreased in the right atria after 4 weeks of maprotiline application. Contrary, the same treatment led to a significant increase in expression of cardiac beta(1)-adrenoceptor in the stressed rats, with no change in the characteristics of beta(2)-adrenoceptor. Our findings might reflect the that molecular mechanisms are underlying factors involved in the development of cardiovascular diseases linked with antidepressant treatment.", journal = "Brazilian Journal of Pharmaceutical Sciences", title = "Maprotiline treatment differentially influences cardiac beta-adrenoreceptors expression under normal and stress conditions", volume = "48", number = "4", pages = "793-799", doi = "10.1590/S1984-82502012000400023" }
Spasojević, N., Jovanović, P.,& Dronjak, S.. (2012). Maprotiline treatment differentially influences cardiac beta-adrenoreceptors expression under normal and stress conditions. in Brazilian Journal of Pharmaceutical Sciences, 48(4), 793-799. https://doi.org/10.1590/S1984-82502012000400023
Spasojević N, Jovanović P, Dronjak S. Maprotiline treatment differentially influences cardiac beta-adrenoreceptors expression under normal and stress conditions. in Brazilian Journal of Pharmaceutical Sciences. 2012;48(4):793-799. doi:10.1590/S1984-82502012000400023 .
Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Maprotiline treatment differentially influences cardiac beta-adrenoreceptors expression under normal and stress conditions" in Brazilian Journal of Pharmaceutical Sciences, 48, no. 4 (2012):793-799, https://doi.org/10.1590/S1984-82502012000400023 . .