Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase

Abstract

Inhibitory effects of five organophosphorus pesticides (diazinon, malathion, chlorpyrifos, azinphosmethyl and phorate) and their oxo-analogs on human myeloperoxidase (MPO) activity were investigated. While inspecting separately peroxidase and chlorination activity, it was observed that investigated OPs affect peroxidase activity, but not chlorination activity. Among investigated pesticides, malathion and malaoxon have showed the highest power to inhibit MPO peroxidase activity With IC50 values of the order of 3 x 10(-7) and 5 x 10(-9) M, respectively. It was proposed that inhibition trend is rendered by molecular structure which invokes steric hindrance for OPs interaction with MPO active center responsible for peroxidase activity. In addition, it was concluded that physiological function of MPO is not affected by any of the investigated OPs. (C) 2013 Elsevier Inc. All rights reserved.