Potential clinical significance of ER beta ON promoter methylation in sporadic breast cancer
Нема приказа
Аутори
Božović, Ana M.Markićević, Milan
Dimitrijević, Bogomir B.
Jovanović-Ćupić, Snežana P.
Krajnović, Milena M.
Lukić, Silvana
Mandušić, Vesna
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
The aim of the study was to assess how hypermethylation of the ON promoter of the estrogen receptor beta (ER beta) gene affects its expression (at the mRNA and protein level) and to correlate these with some clinical and histopathological parameters. A total of 131 samples of frozen breast cancer tissue was analyzed. A custom-designed, two-step PCR method was used to measure the methylation index of the ER beta gene ON promoter region. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed to quantify mRNA of the ER beta 1 isoform, while ER beta 1 protein was determined using the Western blot method. There was a significant difference in the methylation index of the ER beta gene ON promoter between the groups of patients with negative and positive axillary lymph node status (P = 0.03). In addition, the methylation index of the ON promoter was positively correlated with estrogen receptor alfa (ER alpha) protein levels (q = 0.31, P = 0.02). There was a signi...ficant difference in the methylation index of the ON promoter between the progesterone receptor (PR)-negative and PR-positive groups of patients (P = 0.01). ER beta 1 protein levels were negatively correlated with ER alpha protein (q = -0.27, P LT 0.01). The methylation index of the ON promoter could be a more reliable additional parameter for prediction and/or prognosis in breast cancer than ER beta 1-mRNA and/or protein levels.
Кључне речи:
Metastases / Estrogen receptor alpha / Estrogen receptor beta / Progesterone receptor / Methylation / Quantitative PCRИзвор:
Medical Oncology, 2013, 30, 3Финансирање / пројекти:
- Молекуларне детерминанте за дизајн тумор маркера (RS-MESTD-Basic Research (BR or ON)-173049)
- Молекуларни биомаркери карцинома дојке и промена њиховог значаја у зависности од периода праћења тока болести (RS-MESTD-Basic Research (BR or ON)-175068)
DOI: 10.1007/s12032-013-0642-4
ISSN: 1357-0560
PubMed: 23794253
WoS: 000323662900043
Scopus: 2-s2.0-84879036442
Колекције
Институција/група
VinčaTY - JOUR AU - Božović, Ana M. AU - Markićević, Milan AU - Dimitrijević, Bogomir B. AU - Jovanović-Ćupić, Snežana P. AU - Krajnović, Milena M. AU - Lukić, Silvana AU - Mandušić, Vesna PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5647 AB - The aim of the study was to assess how hypermethylation of the ON promoter of the estrogen receptor beta (ER beta) gene affects its expression (at the mRNA and protein level) and to correlate these with some clinical and histopathological parameters. A total of 131 samples of frozen breast cancer tissue was analyzed. A custom-designed, two-step PCR method was used to measure the methylation index of the ER beta gene ON promoter region. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed to quantify mRNA of the ER beta 1 isoform, while ER beta 1 protein was determined using the Western blot method. There was a significant difference in the methylation index of the ER beta gene ON promoter between the groups of patients with negative and positive axillary lymph node status (P = 0.03). In addition, the methylation index of the ON promoter was positively correlated with estrogen receptor alfa (ER alpha) protein levels (q = 0.31, P = 0.02). There was a significant difference in the methylation index of the ON promoter between the progesterone receptor (PR)-negative and PR-positive groups of patients (P = 0.01). ER beta 1 protein levels were negatively correlated with ER alpha protein (q = -0.27, P LT 0.01). The methylation index of the ON promoter could be a more reliable additional parameter for prediction and/or prognosis in breast cancer than ER beta 1-mRNA and/or protein levels. T2 - Medical Oncology T1 - Potential clinical significance of ER beta ON promoter methylation in sporadic breast cancer VL - 30 IS - 3 DO - 10.1007/s12032-013-0642-4 ER -
@article{ author = "Božović, Ana M. and Markićević, Milan and Dimitrijević, Bogomir B. and Jovanović-Ćupić, Snežana P. and Krajnović, Milena M. and Lukić, Silvana and Mandušić, Vesna", year = "2013", abstract = "The aim of the study was to assess how hypermethylation of the ON promoter of the estrogen receptor beta (ER beta) gene affects its expression (at the mRNA and protein level) and to correlate these with some clinical and histopathological parameters. A total of 131 samples of frozen breast cancer tissue was analyzed. A custom-designed, two-step PCR method was used to measure the methylation index of the ER beta gene ON promoter region. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed to quantify mRNA of the ER beta 1 isoform, while ER beta 1 protein was determined using the Western blot method. There was a significant difference in the methylation index of the ER beta gene ON promoter between the groups of patients with negative and positive axillary lymph node status (P = 0.03). In addition, the methylation index of the ON promoter was positively correlated with estrogen receptor alfa (ER alpha) protein levels (q = 0.31, P = 0.02). There was a significant difference in the methylation index of the ON promoter between the progesterone receptor (PR)-negative and PR-positive groups of patients (P = 0.01). ER beta 1 protein levels were negatively correlated with ER alpha protein (q = -0.27, P LT 0.01). The methylation index of the ON promoter could be a more reliable additional parameter for prediction and/or prognosis in breast cancer than ER beta 1-mRNA and/or protein levels.", journal = "Medical Oncology", title = "Potential clinical significance of ER beta ON promoter methylation in sporadic breast cancer", volume = "30", number = "3", doi = "10.1007/s12032-013-0642-4" }
Božović, A. M., Markićević, M., Dimitrijević, B. B., Jovanović-Ćupić, S. P., Krajnović, M. M., Lukić, S.,& Mandušić, V.. (2013). Potential clinical significance of ER beta ON promoter methylation in sporadic breast cancer. in Medical Oncology, 30(3). https://doi.org/10.1007/s12032-013-0642-4
Božović AM, Markićević M, Dimitrijević BB, Jovanović-Ćupić SP, Krajnović MM, Lukić S, Mandušić V. Potential clinical significance of ER beta ON promoter methylation in sporadic breast cancer. in Medical Oncology. 2013;30(3). doi:10.1007/s12032-013-0642-4 .
Božović, Ana M., Markićević, Milan, Dimitrijević, Bogomir B., Jovanović-Ćupić, Snežana P., Krajnović, Milena M., Lukić, Silvana, Mandušić, Vesna, "Potential clinical significance of ER beta ON promoter methylation in sporadic breast cancer" in Medical Oncology, 30, no. 3 (2013), https://doi.org/10.1007/s12032-013-0642-4 . .