Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells
Аутори
Korićanac, LelaŽakula, Jelena
Keta, Otilija D.
Cirrone, Giuseppe Antonio Pablo
Cuttone, Giacomo
Ristić-Fira, Aleksandra
Petrović, Ivan M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.
Кључне речи:
melanoma / carbon ion / apoptosis / p53 / Bax/Bcl-2 ratio / caspase-3Извор:
Nuclear technology and radiation protection, 2013, 28, 2, 195-203Финансирање / пројекти:
- Радиосензитивност хуманог генома (RS-MESTD-Basic Research (BR or ON)-173046)
- Физика високих енергија са детектором CMS (RS-MESTD-Basic Research (BR or ON)-171019)
- Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Catania, Italy
DOI: 10.2298/NTRP1302195K
ISSN: 1451-3994
WoS: 000322101900011
Scopus: 2-s2.0-84881000946
Колекције
Институција/група
VinčaTY - JOUR AU - Korićanac, Lela AU - Žakula, Jelena AU - Keta, Otilija D. AU - Cirrone, Giuseppe Antonio Pablo AU - Cuttone, Giacomo AU - Ristić-Fira, Aleksandra AU - Petrović, Ivan M. PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5593 AB - This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation. T2 - Nuclear technology and radiation protection T1 - Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells VL - 28 IS - 2 SP - 195 EP - 203 DO - 10.2298/NTRP1302195K ER -
@article{ author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.", year = "2013", abstract = "This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.", journal = "Nuclear technology and radiation protection", title = "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells", volume = "28", number = "2", pages = "195-203", doi = "10.2298/NTRP1302195K" }
Korićanac, L., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2013). Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection, 28(2), 195-203. https://doi.org/10.2298/NTRP1302195K
Korićanac L, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection. 2013;28(2):195-203. doi:10.2298/NTRP1302195K .
Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells" in Nuclear technology and radiation protection, 28, no. 2 (2013):195-203, https://doi.org/10.2298/NTRP1302195K . .