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dc.creatorTepavčević, Snežana
dc.creatorMilutinovic, D. V.
dc.creatorMacut, D.
dc.creatorStanišić, Jelena
dc.creatorNikolic, M.
dc.creatorBozic-Antic, I.
dc.creatorRodaljevic, S.
dc.creatorBjekic-Macut, J.
dc.creatorMatić, Gordana
dc.creatorKorićanac, Goran
dc.date.accessioned2018-03-01T16:08:43Z
dc.date.available2018-03-01T16:08:43Z
dc.date.issued2015
dc.identifier.issn0947-7349 (print)
dc.identifier.issn1439-3646 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/553
dc.description.abstractNitric oxide synthases (NOSs) and Na+/K+-ATPase are enzymes essential for regular functioning of the heart. Since both enzymes are under insulin and androgen regulation and since insulin action and androgen level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric oxide (NO) production and sodium/potassium transport would be deteriorated in PCOS. To test our hypothesis we introduced animal model of PCOS based on dihydrotestosterone (DHT) treatment of female Wistar rats and analyzed protein expression, phosphorylation or subcellular localization of endothelial NOS (eNOS), inducible NOS (iNOS) and alpha subunits of Na+/K+-ATPase in the heart. Obtained results indicate that DHT treatment significantly decreased cardiac eNOS protein level and activating phosphorylation at serine 1177, while inhibitory phosphorylation at threonine 495 was increased. In contrast to expression of eNOS, iNOS protein level in the heart of DHT-treated rats was significantly elevated. Furthermore, cardiac protein level of alpha 1 subunit of the ATPase, as well as its plasma membrane content, were decreased in rats with PCOS. In line with this, alpha 2 subunit protein level in fraction of plasma membranes was also significantly below control level. In conclusion, DHT treatment impaired effectiveness of NOSs and Na+/K+-ATPase in the female rat heart. Regarding the importance of NO production and sodium/potassium transport in the cardiac contraction and blood flow regulation, it implicates strong consequences of PCOS for heart functioning.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS//
dc.rightsrestrictedAccessen
dc.sourceExperimental and Clinical Endocrinology and Diabetesen
dc.subjectpolycystic ovary syndromeen
dc.subjectdihydrotestosteroneen
dc.subjectnitric oxide synthaseen
dc.subjectNa+en
dc.subjectK+-ATPaseen
dc.subjecthearten
dc.titleCardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosteroneen
dc.typearticleen
dcterms.abstractМилутиновиц, Д. В.; Мацут, Д.; Станишић Јелена; Тепавчевић Снежана; Корићанац Горан; Бозиц-Aнтиц, И.; Бјекиц-Мацут, Ј.; Родаљевиц, С.; Николиц, М.; Матиц, Г.;
dc.citation.volume123
dc.citation.issue5
dc.citation.spage303
dc.citation.epage307
dc.identifier.wos000354761400007
dc.identifier.doi10.1055/s-0035-1548929
dc.citation.rankM23
dc.identifier.pmid25988879
dc.identifier.scopus2-s2.0-84930210756


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