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Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone

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Authors
Tepavčević, Snežana
Milutinovic, D. V.
Macut, D.
Stanišić, Jelena
Nikolic, M.
Bozic-Antic, I.
Rodaljevic, S.
Bjekic-Macut, J.
Matić, Gordana
Korićanac, Goran
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Abstract
Nitric oxide synthases (NOSs) and Na+/K+-ATPase are enzymes essential for regular functioning of the heart. Since both enzymes are under insulin and androgen regulation and since insulin action and androgen level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric oxide (NO) production and sodium/potassium transport would be deteriorated in PCOS. To test our hypothesis we introduced animal model of PCOS based on dihydrotestosterone (DHT) treatment of female Wistar rats and analyzed protein expression, phosphorylation or subcellular localization of endothelial NOS (eNOS), inducible NOS (iNOS) and alpha subunits of Na+/K+-ATPase in the heart. Obtained results indicate that DHT treatment significantly decreased cardiac eNOS protein level and activating phosphorylation at serine 1177, while inhibitory phosphorylation at threonine 495 was increased. In contrast to expression of eNOS, iNOS protein level in the heart of DHT-treated rats was significantly el...evated. Furthermore, cardiac protein level of alpha 1 subunit of the ATPase, as well as its plasma membrane content, were decreased in rats with PCOS. In line with this, alpha 2 subunit protein level in fraction of plasma membranes was also significantly below control level. In conclusion, DHT treatment impaired effectiveness of NOSs and Na+/K+-ATPase in the female rat heart. Regarding the importance of NO production and sodium/potassium transport in the cardiac contraction and blood flow regulation, it implicates strong consequences of PCOS for heart functioning.

Keywords:
polycystic ovary syndrome / dihydrotestosterone / nitric oxide synthase / Na+ / K+-ATPase / heart
Source:
Experimental and Clinical Endocrinology and Diabetes, 2015, 123, 5, 303-307
Projects:
  • Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-41009)

DOI: 10.1055/s-0035-1548929

ISSN: 0947-7349 (print); 1439-3646 (electronic)

PubMed: 25988879

WoS: 000354761400007

Scopus: 2-s2.0-84930210756
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http://vinar.vin.bg.ac.rs/handle/123456789/553
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