Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
Нема приказа
Аутори
Matić, GordanaMilutinovic, Danijela Vojnovic
Nestorov, Jelena
Elaković, Ivana
Jovanovic, Sanja Manitasevic
Perisic, Tatjana
Dunderski, Jadranka
Damjanović, Svetozar S.
Knežević, Goran
Spiric, Zeljko
Vermetten, Eric
Savić, Danka A.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the recept...or was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.
Кључне речи:
Glucocorticoid receptor / Peripheral blood mononuclear cells / PTSD / Resilience to PTSD / War traumaИзвор:
Progress in Neuro-psychopharmacology and Biological Psychiatry, 2013, 43, 238-245Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-41009)
- Идентификација, мерење и развој когнитивних и емоционалних компетенција важних друштву оријентисаном на европске интеграције (RS-179018)
- European Commission [INCO-CT-2004-509213]
DOI: 10.1016/j.pnpbp.2013.01.005
ISSN: 0278-5846
PubMed: 23333536
WoS: 000317878700031
Scopus: 2-s2.0-84873550690
Колекције
Институција/група
VinčaTY - JOUR AU - Matić, Gordana AU - Milutinovic, Danijela Vojnovic AU - Nestorov, Jelena AU - Elaković, Ivana AU - Jovanovic, Sanja Manitasevic AU - Perisic, Tatjana AU - Dunderski, Jadranka AU - Damjanović, Svetozar S. AU - Knežević, Goran AU - Spiric, Zeljko AU - Vermetten, Eric AU - Savić, Danka A. PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5429 AB - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved. T2 - Progress in Neuro-psychopharmacology and Biological Psychiatry T1 - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD VL - 43 SP - 238 EP - 245 DO - 10.1016/j.pnpbp.2013.01.005 ER -
@article{ author = "Matić, Gordana and Milutinovic, Danijela Vojnovic and Nestorov, Jelena and Elaković, Ivana and Jovanovic, Sanja Manitasevic and Perisic, Tatjana and Dunderski, Jadranka and Damjanović, Svetozar S. and Knežević, Goran and Spiric, Zeljko and Vermetten, Eric and Savić, Danka A.", year = "2013", abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.", journal = "Progress in Neuro-psychopharmacology and Biological Psychiatry", title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD", volume = "43", pages = "238-245", doi = "10.1016/j.pnpbp.2013.01.005" }
Matić, G., Milutinovic, D. V., Nestorov, J., Elaković, I., Jovanovic, S. M., Perisic, T., Dunderski, J., Damjanović, S. S., Knežević, G., Spiric, Z., Vermetten, E.,& Savić, D. A.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-psychopharmacology and Biological Psychiatry, 43, 238-245. https://doi.org/10.1016/j.pnpbp.2013.01.005
Matić G, Milutinovic DV, Nestorov J, Elaković I, Jovanovic SM, Perisic T, Dunderski J, Damjanović SS, Knežević G, Spiric Z, Vermetten E, Savić DA. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-psychopharmacology and Biological Psychiatry. 2013;43:238-245. doi:10.1016/j.pnpbp.2013.01.005 .
Matić, Gordana, Milutinovic, Danijela Vojnovic, Nestorov, Jelena, Elaković, Ivana, Jovanovic, Sanja Manitasevic, Perisic, Tatjana, Dunderski, Jadranka, Damjanović, Svetozar S., Knežević, Goran, Spiric, Zeljko, Vermetten, Eric, Savić, Danka A., "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-psychopharmacology and Biological Psychiatry, 43 (2013):238-245, https://doi.org/10.1016/j.pnpbp.2013.01.005 . .