Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test
Apstrakt
Organothiophosphorous pesticides diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate, have been indirectly electrochemically oxidized in aqueous media using anodically evolved Cl-2, Br-2 or I-2 as a pre-step for their detection via acetylcholinesterase-based test. The presence of single oxidation product, corresponding oxo-form, was confirmed by UPLC analysis, as well as its stability with respect to hydrolysis. Comparing different halogens, the best results were obtained using Br-2 as the oxidant due to high reactivity of HOBr, which is formed upon chemical reaction of anodically formed Br-2 with water. Limits of detection of five analyzed pesticides were lowered upon indirect electrochemical oxidation with Br-2 for two orders of magnitude or more, comparing to unoxidized parental thio-forms. In fact, the lowest possible detection limits for all five pesticides using proposed analytical procedure were achieved, as being determined by detection limits of corresponding oxo fo...rms. Comparison of here proposed electrochemical oxidation pre-step with earlier reported ones is provided and discussed. (c) 2012 Elsevier Inc. All rights reserved.
Ključne reči:
Organophosphate / Pesticide / Oxidation / Halogen / acetylcholinesterase / MyeloperoxidaseIzvor:
Pesticide Biochemistry and Physiology, 2012, 104, 3, 236-242Finansiranje / projekti:
- Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (RS-MESTD-Basic Research (BR or ON)-172023)
DOI: 10.1016/j.pestbp.2012.09.004
ISSN: 0048-3575; 1095-9939
WoS: 000313599900011
Scopus: 2-s2.0-84870301511
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Lazarević-Pašti, Tamara AU - Bondžić, Aleksandra M. AU - Pašti, Igor A. AU - Vasić, Vesna M. PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5263 AB - Organothiophosphorous pesticides diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate, have been indirectly electrochemically oxidized in aqueous media using anodically evolved Cl-2, Br-2 or I-2 as a pre-step for their detection via acetylcholinesterase-based test. The presence of single oxidation product, corresponding oxo-form, was confirmed by UPLC analysis, as well as its stability with respect to hydrolysis. Comparing different halogens, the best results were obtained using Br-2 as the oxidant due to high reactivity of HOBr, which is formed upon chemical reaction of anodically formed Br-2 with water. Limits of detection of five analyzed pesticides were lowered upon indirect electrochemical oxidation with Br-2 for two orders of magnitude or more, comparing to unoxidized parental thio-forms. In fact, the lowest possible detection limits for all five pesticides using proposed analytical procedure were achieved, as being determined by detection limits of corresponding oxo forms. Comparison of here proposed electrochemical oxidation pre-step with earlier reported ones is provided and discussed. (c) 2012 Elsevier Inc. All rights reserved. T2 - Pesticide Biochemistry and Physiology T1 - Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test VL - 104 IS - 3 SP - 236 EP - 242 DO - 10.1016/j.pestbp.2012.09.004 ER -
@article{ author = "Lazarević-Pašti, Tamara and Bondžić, Aleksandra M. and Pašti, Igor A. and Vasić, Vesna M.", year = "2012", abstract = "Organothiophosphorous pesticides diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate, have been indirectly electrochemically oxidized in aqueous media using anodically evolved Cl-2, Br-2 or I-2 as a pre-step for their detection via acetylcholinesterase-based test. The presence of single oxidation product, corresponding oxo-form, was confirmed by UPLC analysis, as well as its stability with respect to hydrolysis. Comparing different halogens, the best results were obtained using Br-2 as the oxidant due to high reactivity of HOBr, which is formed upon chemical reaction of anodically formed Br-2 with water. Limits of detection of five analyzed pesticides were lowered upon indirect electrochemical oxidation with Br-2 for two orders of magnitude or more, comparing to unoxidized parental thio-forms. In fact, the lowest possible detection limits for all five pesticides using proposed analytical procedure were achieved, as being determined by detection limits of corresponding oxo forms. Comparison of here proposed electrochemical oxidation pre-step with earlier reported ones is provided and discussed. (c) 2012 Elsevier Inc. All rights reserved.", journal = "Pesticide Biochemistry and Physiology", title = "Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test", volume = "104", number = "3", pages = "236-242", doi = "10.1016/j.pestbp.2012.09.004" }
Lazarević-Pašti, T., Bondžić, A. M., Pašti, I. A.,& Vasić, V. M.. (2012). Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test. in Pesticide Biochemistry and Physiology, 104(3), 236-242. https://doi.org/10.1016/j.pestbp.2012.09.004
Lazarević-Pašti T, Bondžić AM, Pašti IA, Vasić VM. Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test. in Pesticide Biochemistry and Physiology. 2012;104(3):236-242. doi:10.1016/j.pestbp.2012.09.004 .
Lazarević-Pašti, Tamara, Bondžić, Aleksandra M., Pašti, Igor A., Vasić, Vesna M., "Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test" in Pesticide Biochemistry and Physiology, 104, no. 3 (2012):236-242, https://doi.org/10.1016/j.pestbp.2012.09.004 . .