Antioxidant activity of propolis extracts from Serbia: A polarographic approach
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Potkonjak, Nebojša I.Veselinović, Dragan S.
Novaković, Miroslav M.
Gorjanovic, Stanislava Z.
Pezo, Lato
Sužnjevicć, Desanka Ž.
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Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correl...ated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. (C) 2012 Elsevier Ltd. All rights reserved.
Keywords:
Propolis extract / Antioxidant activity / Hydrogen peroxide / PolarographySource:
Food and Chemical Toxicology, 2012, 50, 10, 3614-3618Funding / projects:
- Dynamics of nonlinear physicochemical and biochemical systems with modeling and predicting of their behavior under nonequilibrium conditions (RS-MESTD-Basic Research (BR or ON)-172015)
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
DOI: 10.1016/j.fct.2012.07.029
ISSN: 0278-6915; 1873-6351
PubMed: 22842121
WoS: 000309897100032
Scopus: 2-s2.0-84865518003
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VinčaTY - JOUR AU - Potkonjak, Nebojša I. AU - Veselinović, Dragan S. AU - Novaković, Miroslav M. AU - Gorjanovic, Stanislava Z. AU - Pezo, Lato AU - Sužnjevicć, Desanka Ž. PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5101 AB - Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. (C) 2012 Elsevier Ltd. All rights reserved. T2 - Food and Chemical Toxicology T1 - Antioxidant activity of propolis extracts from Serbia: A polarographic approach VL - 50 IS - 10 SP - 3614 EP - 3618 DO - 10.1016/j.fct.2012.07.029 ER -
@article{ author = "Potkonjak, Nebojša I. and Veselinović, Dragan S. and Novaković, Miroslav M. and Gorjanovic, Stanislava Z. and Pezo, Lato and Sužnjevicć, Desanka Ž.", year = "2012", abstract = "Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. (C) 2012 Elsevier Ltd. All rights reserved.", journal = "Food and Chemical Toxicology", title = "Antioxidant activity of propolis extracts from Serbia: A polarographic approach", volume = "50", number = "10", pages = "3614-3618", doi = "10.1016/j.fct.2012.07.029" }
Potkonjak, N. I., Veselinović, D. S., Novaković, M. M., Gorjanovic, S. Z., Pezo, L.,& Sužnjevicć, D. Ž.. (2012). Antioxidant activity of propolis extracts from Serbia: A polarographic approach. in Food and Chemical Toxicology, 50(10), 3614-3618. https://doi.org/10.1016/j.fct.2012.07.029
Potkonjak NI, Veselinović DS, Novaković MM, Gorjanovic SZ, Pezo L, Sužnjevicć DŽ. Antioxidant activity of propolis extracts from Serbia: A polarographic approach. in Food and Chemical Toxicology. 2012;50(10):3614-3618. doi:10.1016/j.fct.2012.07.029 .
Potkonjak, Nebojša I., Veselinović, Dragan S., Novaković, Miroslav M., Gorjanovic, Stanislava Z., Pezo, Lato, Sužnjevicć, Desanka Ž., "Antioxidant activity of propolis extracts from Serbia: A polarographic approach" in Food and Chemical Toxicology, 50, no. 10 (2012):3614-3618, https://doi.org/10.1016/j.fct.2012.07.029 . .