Interaction of Gold Nanoparticles with Rat Brain Synaptosomal Plasma Membrane Na+/K+ - Atpase and Mg2+-Atpase
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The aim of the work was to investigate the interaction between borate capped gold nanoparticles (NPs) and the rat brain synaptosomal plasma membranes (SPM), as well as the effects of these NPs on SPM Na+/K+ - ATPase and Mg2+-ATPase activity. The changes in the UV-vis spectra of NPs and SPM assembly suggested the agglomeration and precipitation of NPs. FTIR measurements indicated that both protein -SH and -NH2 groups and positively charged membrane fragments were implicated in the adhesion of SPM to the surface of NPs. AFM showed an increase in the particularization of the SPM material after mixing with gold NPs. Influence of gold NPs on Na+/K+-ATPase and Mg2+-ATPase activity was investigated as the function of NPs and protein concentration, preincubation time and also in the presence of various concentrations of ouabain, the specific enzyme inhibitor. NPs induced the stimulation of Na+/K+-ATPase activity for more than 100%, since Mg2+-ATPase activity reminded unaffected. We propose tha...t this stimulation of enzyme activity was a consequence of an increase of the active surface of membranes.
Кључне речи:Gold nanoparticles / Na+/K+-ATPase / Mg2+-ATPase / synaptosomal plasma membranes / biofunctionalization
Извор:Digest Journal of Nanomaterials and Biostructures, 2012, 7, 2, 423-433
ISSN: 1842-3582 (print)