Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats
Само за регистроване кориснике
2012
Аутори
Gavrilović, LjubicaStojiljković, Vesna
Kasapović, Jelena
Pejić, Snežana
Todorović, Ana
Pajović, Snežana B.
Dronjak, Slađana
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, C...FR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions.
Кључне речи:
acute immobilization stress / adrenal medulla / catecholamine / chronic stress / gene expressionИзвор:
Acta Veterinaria, Beograd, 2012, 62, 2-3, 151-169Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- Акутни коронарни синдром: истраживање вулнерабилности (плака, крви и миокарда), оптимално лечење и одређивање прогностичких фактора (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41022)
- Молекуларни механизми патофизиолошких промена у ћелијама централног нервног система и периферног ткива код сисара (RS-MESTD-Basic Research (BR or ON)-173044)
DOI: 10.2298/AVB1203151G
ISSN: 0567-8315
WoS: 000305775000003
Scopus: 2-s2.0-84864615859
Колекције
Институција/група
VinčaTY - JOUR AU - Gavrilović, Ljubica AU - Stojiljković, Vesna AU - Kasapović, Jelena AU - Pejić, Snežana AU - Todorović, Ana AU - Pajović, Snežana B. AU - Dronjak, Slađana PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4915 AB - In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, CFR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions. T2 - Acta Veterinaria, Beograd T1 - Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats VL - 62 IS - 2-3 SP - 151 EP - 169 DO - 10.2298/AVB1203151G ER -
@article{ author = "Gavrilović, Ljubica and Stojiljković, Vesna and Kasapović, Jelena and Pejić, Snežana and Todorović, Ana and Pajović, Snežana B. and Dronjak, Slađana", year = "2012", abstract = "In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, CFR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions.", journal = "Acta Veterinaria, Beograd", title = "Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats", volume = "62", number = "2-3", pages = "151-169", doi = "10.2298/AVB1203151G" }
Gavrilović, L., Stojiljković, V., Kasapović, J., Pejić, S., Todorović, A., Pajović, S. B.,& Dronjak, S.. (2012). Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats. in Acta Veterinaria, Beograd, 62(2-3), 151-169. https://doi.org/10.2298/AVB1203151G
Gavrilović L, Stojiljković V, Kasapović J, Pejić S, Todorović A, Pajović SB, Dronjak S. Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats. in Acta Veterinaria, Beograd. 2012;62(2-3):151-169. doi:10.2298/AVB1203151G .
Gavrilović, Ljubica, Stojiljković, Vesna, Kasapović, Jelena, Pejić, Snežana, Todorović, Ana, Pajović, Snežana B., Dronjak, Slađana, "Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats" in Acta Veterinaria, Beograd, 62, no. 2-3 (2012):151-169, https://doi.org/10.2298/AVB1203151G . .