Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts
Нема приказа
Аутори
Nastasijević, Branislav J.Lazarević-Pašti, Tamara
Dimitrijević-Branković, Suzana I.
Pašti, Igor A.
Vujačić, Ana V.
Joksić, Gordana
Vasić, Vesna M.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constitu...ents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC). (C) 2012 Elsevier B.V. All rights reserved.
Кључне речи:
Cyclic voltammetry / DPPH test / Gentiana lutea / Gentiopicoside / Myeloperoxidase / UPLCИзвор:
Journal of Pharmaceutical and Biomedical Analysis, 2012, 66, 191-196Финансирање / пројекти:
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-MESTD-Basic Research (BR or ON)-172023)
DOI: 10.1016/j.jpba.2012.03.052
ISSN: 0731-7085
PubMed: 22521634
WoS: 000304843600023
Scopus: 2-s2.0-84861221232
Колекције
Институција/група
VinčaTY - JOUR AU - Nastasijević, Branislav J. AU - Lazarević-Pašti, Tamara AU - Dimitrijević-Branković, Suzana I. AU - Pašti, Igor A. AU - Vujačić, Ana V. AU - Joksić, Gordana AU - Vasić, Vesna M. PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4873 AB - The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC). (C) 2012 Elsevier B.V. All rights reserved. T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts VL - 66 SP - 191 EP - 196 DO - 10.1016/j.jpba.2012.03.052 ER -
@article{ author = "Nastasijević, Branislav J. and Lazarević-Pašti, Tamara and Dimitrijević-Branković, Suzana I. and Pašti, Igor A. and Vujačić, Ana V. and Joksić, Gordana and Vasić, Vesna M.", year = "2012", abstract = "The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC). (C) 2012 Elsevier B.V. All rights reserved.", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts", volume = "66", pages = "191-196", doi = "10.1016/j.jpba.2012.03.052" }
Nastasijević, B. J., Lazarević-Pašti, T., Dimitrijević-Branković, S. I., Pašti, I. A., Vujačić, A. V., Joksić, G.,& Vasić, V. M.. (2012). Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts. in Journal of Pharmaceutical and Biomedical Analysis, 66, 191-196. https://doi.org/10.1016/j.jpba.2012.03.052
Nastasijević BJ, Lazarević-Pašti T, Dimitrijević-Branković SI, Pašti IA, Vujačić AV, Joksić G, Vasić VM. Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts. in Journal of Pharmaceutical and Biomedical Analysis. 2012;66:191-196. doi:10.1016/j.jpba.2012.03.052 .
Nastasijević, Branislav J., Lazarević-Pašti, Tamara, Dimitrijević-Branković, Suzana I., Pašti, Igor A., Vujačić, Ana V., Joksić, Gordana, Vasić, Vesna M., "Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts" in Journal of Pharmaceutical and Biomedical Analysis, 66 (2012):191-196, https://doi.org/10.1016/j.jpba.2012.03.052 . .