Chronic Stress Decreases Availability of Heat Shock Proteins to Glucocorticoid Receptor in Response to Novel Acute Stress in Wistar Rat Hypothalamus
Abstract
Chronic psychosocial isolation (CPSI) is known to cause several maladaptive changes in the limbic brain structures, which regulate the hypothalamic-pituitary-adrenal (HPA) axis activity. In this study, we focused our investigation on CPSI effects in the hypothalamus (HT) since it is a major driver of HPA axis activity. We also investigated whether the exposure to CPSI could alter the response to subsequent acute stress (30-min immobilization). In the HT, we followed cytosolic and nuclear levels of the glucocorticoid receptor (GR), as a mediator of HPA axis feedback inhibition, and its chaperones, the heat shock proteins (HSPs), hsp70 and hsp90. The CPSI did not cause any changes in either GR or HSPs levels. However, we observed increase of the GR and hsp70 in both HT cellular compartments as a response of na LT ve rats to acute stress, whereas the response of CPSI rats to acute stress was associated with elevation of the GR in the cytosol and decrease of HSPs in the nucleus. Thus, our ...data indicated reduced availability of HSPs to GR in both cytosol and nucleus of the HT under acute stress of CPSI animals, and therefore, pointed out to potentially negative effects of CPSI on GR function in the HT.
Keywords:
Chronic stress / Hypothalamus / Glucocorticoid receptor / Heat shock proteinsSource:
Cellular and Molecular Neurobiology, 2012, 32, 4, 625-632Funding / projects:
- Defining a cluster of molecular biomarkers for improved diagnostics and therapy of mood disorders (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.1007/s10571-012-9811-9
ISSN: 0272-4340
PubMed: 22350213
WoS: 000303410000015
Scopus: 2-s2.0-84864674770
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VinčaTY - JOUR AU - Simić, Iva AU - Mitić, Miloš AU - Đorđević, Jelena D. AU - Radojčić, Marija AU - Adžić, Miroslav PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4825 AB - Chronic psychosocial isolation (CPSI) is known to cause several maladaptive changes in the limbic brain structures, which regulate the hypothalamic-pituitary-adrenal (HPA) axis activity. In this study, we focused our investigation on CPSI effects in the hypothalamus (HT) since it is a major driver of HPA axis activity. We also investigated whether the exposure to CPSI could alter the response to subsequent acute stress (30-min immobilization). In the HT, we followed cytosolic and nuclear levels of the glucocorticoid receptor (GR), as a mediator of HPA axis feedback inhibition, and its chaperones, the heat shock proteins (HSPs), hsp70 and hsp90. The CPSI did not cause any changes in either GR or HSPs levels. However, we observed increase of the GR and hsp70 in both HT cellular compartments as a response of na LT ve rats to acute stress, whereas the response of CPSI rats to acute stress was associated with elevation of the GR in the cytosol and decrease of HSPs in the nucleus. Thus, our data indicated reduced availability of HSPs to GR in both cytosol and nucleus of the HT under acute stress of CPSI animals, and therefore, pointed out to potentially negative effects of CPSI on GR function in the HT. T2 - Cellular and Molecular Neurobiology T1 - Chronic Stress Decreases Availability of Heat Shock Proteins to Glucocorticoid Receptor in Response to Novel Acute Stress in Wistar Rat Hypothalamus VL - 32 IS - 4 SP - 625 EP - 632 DO - 10.1007/s10571-012-9811-9 ER -
@article{ author = "Simić, Iva and Mitić, Miloš and Đorđević, Jelena D. and Radojčić, Marija and Adžić, Miroslav", year = "2012", abstract = "Chronic psychosocial isolation (CPSI) is known to cause several maladaptive changes in the limbic brain structures, which regulate the hypothalamic-pituitary-adrenal (HPA) axis activity. In this study, we focused our investigation on CPSI effects in the hypothalamus (HT) since it is a major driver of HPA axis activity. We also investigated whether the exposure to CPSI could alter the response to subsequent acute stress (30-min immobilization). In the HT, we followed cytosolic and nuclear levels of the glucocorticoid receptor (GR), as a mediator of HPA axis feedback inhibition, and its chaperones, the heat shock proteins (HSPs), hsp70 and hsp90. The CPSI did not cause any changes in either GR or HSPs levels. However, we observed increase of the GR and hsp70 in both HT cellular compartments as a response of na LT ve rats to acute stress, whereas the response of CPSI rats to acute stress was associated with elevation of the GR in the cytosol and decrease of HSPs in the nucleus. Thus, our data indicated reduced availability of HSPs to GR in both cytosol and nucleus of the HT under acute stress of CPSI animals, and therefore, pointed out to potentially negative effects of CPSI on GR function in the HT.", journal = "Cellular and Molecular Neurobiology", title = "Chronic Stress Decreases Availability of Heat Shock Proteins to Glucocorticoid Receptor in Response to Novel Acute Stress in Wistar Rat Hypothalamus", volume = "32", number = "4", pages = "625-632", doi = "10.1007/s10571-012-9811-9" }
Simić, I., Mitić, M., Đorđević, J. D., Radojčić, M.,& Adžić, M.. (2012). Chronic Stress Decreases Availability of Heat Shock Proteins to Glucocorticoid Receptor in Response to Novel Acute Stress in Wistar Rat Hypothalamus. in Cellular and Molecular Neurobiology, 32(4), 625-632. https://doi.org/10.1007/s10571-012-9811-9
Simić I, Mitić M, Đorđević JD, Radojčić M, Adžić M. Chronic Stress Decreases Availability of Heat Shock Proteins to Glucocorticoid Receptor in Response to Novel Acute Stress in Wistar Rat Hypothalamus. in Cellular and Molecular Neurobiology. 2012;32(4):625-632. doi:10.1007/s10571-012-9811-9 .
Simić, Iva, Mitić, Miloš, Đorđević, Jelena D., Radojčić, Marija, Adžić, Miroslav, "Chronic Stress Decreases Availability of Heat Shock Proteins to Glucocorticoid Receptor in Response to Novel Acute Stress in Wistar Rat Hypothalamus" in Cellular and Molecular Neurobiology, 32, no. 4 (2012):625-632, https://doi.org/10.1007/s10571-012-9811-9 . .