Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats
Апстракт
Stress exposure activates the sympathoneural system, resulting in catecholamine release. Chronic stress is associated with development of numerous disorders, including cardiovascular diseases. Here we investigated the expression of mRNAs for catecholamine biosynthetic enzymes tyrosine-hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase, and for beta(1)- and beta(2)-adrenoceptors in the right and left ventricles of rats exposed to chronic unpredictable mild stress. The tyrosine-hydroxylase and dopamine-beta-hydroxylase mRNA levels were not affected by stress, whereas the phenylethanolamine N-methyltransferase mRNA levels significantly increased in both right and left ventricles. No changes in beta(1)-adrenoceptor mRNA levels in either right or left ventricles were observed. At the same time, stress produced a significant increase of beta(2)-adrenoceptor mRNA levels in left ventricles. These results suggest that elevated expression of phenylethanolamine N-me...thyltransferase in both ventricules and beta(2)-adrenoceptor genes in left ventricles could provide a molecular mechanism that leads to altered physiological response, which is important for the organism coping with stress.
Кључне речи:
Chronic stress / Ventricles / Catecholamine enzymes / beta-adrenoceptors / Gene expressionИзвор:
Physiological Research, 2011, 60, S171-S176Финансирање / пројекти:
- Сигнални путеви деловања стероидних хормона и утицај ендогених и егзогених фактора на модулацију процеса у ћелијама сисара (RS-MESTD-MPN2006-2010-143044)
Колекције
Институција/група
VinčaTY - JOUR AU - Spasojević, Nataša AU - Gavrilović, Ljubica AU - Dronjak, Slađana PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4539 AB - Stress exposure activates the sympathoneural system, resulting in catecholamine release. Chronic stress is associated with development of numerous disorders, including cardiovascular diseases. Here we investigated the expression of mRNAs for catecholamine biosynthetic enzymes tyrosine-hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase, and for beta(1)- and beta(2)-adrenoceptors in the right and left ventricles of rats exposed to chronic unpredictable mild stress. The tyrosine-hydroxylase and dopamine-beta-hydroxylase mRNA levels were not affected by stress, whereas the phenylethanolamine N-methyltransferase mRNA levels significantly increased in both right and left ventricles. No changes in beta(1)-adrenoceptor mRNA levels in either right or left ventricles were observed. At the same time, stress produced a significant increase of beta(2)-adrenoceptor mRNA levels in left ventricles. These results suggest that elevated expression of phenylethanolamine N-methyltransferase in both ventricules and beta(2)-adrenoceptor genes in left ventricles could provide a molecular mechanism that leads to altered physiological response, which is important for the organism coping with stress. T2 - Physiological Research T1 - Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats VL - 60 SP - S171 EP - S176 UR - https://hdl.handle.net/21.15107/rcub_vinar_4539 ER -
@article{ author = "Spasojević, Nataša and Gavrilović, Ljubica and Dronjak, Slađana", year = "2011", abstract = "Stress exposure activates the sympathoneural system, resulting in catecholamine release. Chronic stress is associated with development of numerous disorders, including cardiovascular diseases. Here we investigated the expression of mRNAs for catecholamine biosynthetic enzymes tyrosine-hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase, and for beta(1)- and beta(2)-adrenoceptors in the right and left ventricles of rats exposed to chronic unpredictable mild stress. The tyrosine-hydroxylase and dopamine-beta-hydroxylase mRNA levels were not affected by stress, whereas the phenylethanolamine N-methyltransferase mRNA levels significantly increased in both right and left ventricles. No changes in beta(1)-adrenoceptor mRNA levels in either right or left ventricles were observed. At the same time, stress produced a significant increase of beta(2)-adrenoceptor mRNA levels in left ventricles. These results suggest that elevated expression of phenylethanolamine N-methyltransferase in both ventricules and beta(2)-adrenoceptor genes in left ventricles could provide a molecular mechanism that leads to altered physiological response, which is important for the organism coping with stress.", journal = "Physiological Research", title = "Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats", volume = "60", pages = "S171-S176", url = "https://hdl.handle.net/21.15107/rcub_vinar_4539" }
Spasojević, N., Gavrilović, L.,& Dronjak, S.. (2011). Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats. in Physiological Research, 60, S171-S176. https://hdl.handle.net/21.15107/rcub_vinar_4539
Spasojević N, Gavrilović L, Dronjak S. Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats. in Physiological Research. 2011;60:S171-S176. https://hdl.handle.net/21.15107/rcub_vinar_4539 .
Spasojević, Nataša, Gavrilović, Ljubica, Dronjak, Slađana, "Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats" in Physiological Research, 60 (2011):S171-S176, https://hdl.handle.net/21.15107/rcub_vinar_4539 .