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dc.creatorLazarević-Pašti, Tamara
dc.creatorČolović, Mirjana B.
dc.creatorSavić, Jasmina
dc.creatorMomić, Tatjana
dc.creatorVasić, Vesna M.
dc.date.accessioned2018-03-01T21:45:32Z
dc.date.available2018-03-01T21:45:32Z
dc.date.issued2011
dc.identifier.issn0048-3575 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/4332
dc.description.abstractThe aim of the work was to investigate the in vitro oxidation of diazinon and malathion, organophosphorous pesticides (OPs) containing phosphorthioate group, catalyzed by enzyme myeloperoxidase (MPO). The oxidation was performed in the presence of hydrogen peroxide. The products were identified as oxon derivatives (phosphates), where the sulfur atom from thioate group was substituted by an oxygen atom. No hydrolysis products were detected after enzyme - induced oxidation. The oxidation efficiency was controlled using acethylcholinesterase (AChE) bioassay for determination of oxon derivatives concentration. The influence of OPs concentration, incubation time of OPs with MPO, as well as MPO concentration on the yield of oxo forms was investigated. Kinetic constants of MPO in oxidation of malathion and diazinon were estimated. The maximum concentration of oxo forms was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO. (C) 2011 Elsevier Inc. All rights reserved.en
dc.relationMinistry of Science and Technological Development of the Republic of Serbia
dc.rightsrestrictedAccessen
dc.sourcePesticide Biochemistry and Physiologyen
dc.subjectOrganophosphatesen
dc.subjectOxidationen
dc.subjectMyeloperoxidaseen
dc.subjectaceylcholinesteraseen
dc.subjectKinetic constantsen
dc.titleOxidation of diazinon and malathion by myeloperoxidaseen
dc.typearticleen
dcterms.abstractЛазаревић-Пасти Тамара; Чоловић Мирјана; Савић Јасмина; Момић Татјана; Васић Весна М;
dc.citation.volume100
dc.citation.issue2
dc.citation.spage140
dc.citation.epage144
dc.identifier.wos000291192700006
dc.identifier.doi10.1016/j.pestbp.2011.03.001
dc.citation.rankM21
dc.identifier.scopus2-s2.0-79955875646


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