Oxidation of diazinon and malathion by myeloperoxidase
Апстракт
The aim of the work was to investigate the in vitro oxidation of diazinon and malathion, organophosphorous pesticides (OPs) containing phosphorthioate group, catalyzed by enzyme myeloperoxidase (MPO). The oxidation was performed in the presence of hydrogen peroxide. The products were identified as oxon derivatives (phosphates), where the sulfur atom from thioate group was substituted by an oxygen atom. No hydrolysis products were detected after enzyme - induced oxidation. The oxidation efficiency was controlled using acethylcholinesterase (AChE) bioassay for determination of oxon derivatives concentration. The influence of OPs concentration, incubation time of OPs with MPO, as well as MPO concentration on the yield of oxo forms was investigated. Kinetic constants of MPO in oxidation of malathion and diazinon were estimated. The maximum concentration of oxo forms was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO. (C) 2011 Elsevier Inc. All ri...ghts reserved.
Кључне речи:
Organophosphates / Oxidation / Myeloperoxidase / aceylcholinesterase / Kinetic constantsИзвор:
Pesticide Biochemistry and Physiology, 2011, 100, 2, 140-144Финансирање / пројекти:
- Ministry of Science and Technological Development of the Republic of Serbia
DOI: 10.1016/j.pestbp.2011.03.001
ISSN: 0048-3575
WoS: 000291192700006
Scopus: 2-s2.0-79955875646
Колекције
Институција/група
VinčaTY - JOUR AU - Lazarević-Pašti, Tamara AU - Čolović, Mirjana B. AU - Savić, Jasmina AU - Momić, Tatjana AU - Vasić, Vesna M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4332 AB - The aim of the work was to investigate the in vitro oxidation of diazinon and malathion, organophosphorous pesticides (OPs) containing phosphorthioate group, catalyzed by enzyme myeloperoxidase (MPO). The oxidation was performed in the presence of hydrogen peroxide. The products were identified as oxon derivatives (phosphates), where the sulfur atom from thioate group was substituted by an oxygen atom. No hydrolysis products were detected after enzyme - induced oxidation. The oxidation efficiency was controlled using acethylcholinesterase (AChE) bioassay for determination of oxon derivatives concentration. The influence of OPs concentration, incubation time of OPs with MPO, as well as MPO concentration on the yield of oxo forms was investigated. Kinetic constants of MPO in oxidation of malathion and diazinon were estimated. The maximum concentration of oxo forms was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO. (C) 2011 Elsevier Inc. All rights reserved. T2 - Pesticide Biochemistry and Physiology T1 - Oxidation of diazinon and malathion by myeloperoxidase VL - 100 IS - 2 SP - 140 EP - 144 DO - 10.1016/j.pestbp.2011.03.001 ER -
@article{ author = "Lazarević-Pašti, Tamara and Čolović, Mirjana B. and Savić, Jasmina and Momić, Tatjana and Vasić, Vesna M.", year = "2011", abstract = "The aim of the work was to investigate the in vitro oxidation of diazinon and malathion, organophosphorous pesticides (OPs) containing phosphorthioate group, catalyzed by enzyme myeloperoxidase (MPO). The oxidation was performed in the presence of hydrogen peroxide. The products were identified as oxon derivatives (phosphates), where the sulfur atom from thioate group was substituted by an oxygen atom. No hydrolysis products were detected after enzyme - induced oxidation. The oxidation efficiency was controlled using acethylcholinesterase (AChE) bioassay for determination of oxon derivatives concentration. The influence of OPs concentration, incubation time of OPs with MPO, as well as MPO concentration on the yield of oxo forms was investigated. Kinetic constants of MPO in oxidation of malathion and diazinon were estimated. The maximum concentration of oxo forms was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO. (C) 2011 Elsevier Inc. All rights reserved.", journal = "Pesticide Biochemistry and Physiology", title = "Oxidation of diazinon and malathion by myeloperoxidase", volume = "100", number = "2", pages = "140-144", doi = "10.1016/j.pestbp.2011.03.001" }
Lazarević-Pašti, T., Čolović, M. B., Savić, J., Momić, T.,& Vasić, V. M.. (2011). Oxidation of diazinon and malathion by myeloperoxidase. in Pesticide Biochemistry and Physiology, 100(2), 140-144. https://doi.org/10.1016/j.pestbp.2011.03.001
Lazarević-Pašti T, Čolović MB, Savić J, Momić T, Vasić VM. Oxidation of diazinon and malathion by myeloperoxidase. in Pesticide Biochemistry and Physiology. 2011;100(2):140-144. doi:10.1016/j.pestbp.2011.03.001 .
Lazarević-Pašti, Tamara, Čolović, Mirjana B., Savić, Jasmina, Momić, Tatjana, Vasić, Vesna M., "Oxidation of diazinon and malathion by myeloperoxidase" in Pesticide Biochemistry and Physiology, 100, no. 2 (2011):140-144, https://doi.org/10.1016/j.pestbp.2011.03.001 . .