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Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats
dc.creator | Sudar, Emina | |
dc.creator | Dobutović, Branislava | |
dc.creator | Soskić, Sanja S. | |
dc.creator | Mandušić, Vesna | |
dc.creator | Žakula, Zorica | |
dc.creator | Misirkić, Maja | |
dc.creator | Vucicevic, Ljubica | |
dc.creator | Janjetović, Kristina D. | |
dc.creator | Trajković, Vladimir S. | |
dc.creator | Mikhailidis, Dimitri P. | |
dc.creator | Isenović, Esma R. | |
dc.date.accessioned | 2018-03-01T21:44:16Z | |
dc.date.available | 2018-03-01T21:44:16Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1138-7548 | |
dc.identifier.issn | 1877-8755 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/4317 | |
dc.description.abstract | The purpose of this study was to examine the effects of ghrelin on protein kinase B (Akt) and mitogen-activated protein kinase p42/44 (ERK1/2) activation as well as ghrelin effects on inducible nitric oxide (NO) synthase (iNOS; for gene Nos2) activity/expression in rat hearts. Male Wistar rats were treated with ghrelin (0.3 nmol/5 mu l) or an equal volume of phosphate-buffered saline, injected every 24 h into the lateral cerebral ventricle for 5 days and 2 h after the last treatment the animals were sacrificed. Serum NO, l-arginine (l-Arg), and arginase activity were measured spectrophotometrically. For phosphorylation of Akt, ERK1/2, and iNOS protein expression, Western blot method was used. The expression of Nos2 mRNA was measured by the quantitative real-time polymerase chain reaction (qRT-PCR). Treatment with ghrelin significantly increased NO production in serum by 1.4-fold compared with control. The concentration of l-Arg was significantly higher in ghrelin-treated rats than in control while arginase activity was significantly lower in ghrelin-treated than in control hearts. Ghrelin treatment increased phosphorylation of Akt by 1.9-fold and ERK1/2 by 1.6-fold and increased iNOS expression by 2.5-fold compared with control. In addition, ghrelin treatment increased Nos2 gene expression by 2.2-fold as determined by qRT-PCR. These results indicate that ghrelin regulation of iNOS expression/activity is mediated via Akt/ERK1/2 signaling pathway. These results may be relevant to understanding molecular mechanisms underlying direct cardiovascular actions of ghrelin. | en |
dc.relation | Ministry of Science, Republic of Serbia [143030B, 145073] | |
dc.rights | restrictedAccess | en |
dc.source | Journal of Physiology and Biochemistry | en |
dc.subject | Ghrelin | en |
dc.subject | Nitric oxide synthase | en |
dc.subject | Akt | en |
dc.subject | ERK1/2 | en |
dc.title | Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats | en |
dc.type | article | en |
dcterms.abstract | Судар Емина; Соскић Сања; Мандушић Весна; Вуцицевиц, Љубица; Јањетовиц, Кристина; Исеновић Есма; Добутовиц, Бранислава; Закула, Зорица; Мисиркиц, Маја; Трајковиц, Владимир; Микхаилидис, Димитри П.; | |
dc.citation.volume | 67 | |
dc.citation.issue | 2 | |
dc.citation.spage | 195 | |
dc.citation.epage | 204 | |
dc.identifier.wos | 000290772900006 | |
dc.identifier.doi | 10.1007/s13105-010-0063-1 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 21107779 | |
dc.identifier.scopus | 2-s2.0-80053465690 |