Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering
Нема приказа
Аутори
Stanić, VojislavJanaćković, Đorđe T.
Dimitrijević, Suzana I.
Tanaskovic, Sladjana B.
Mitrić, Miodrag
Pavlović, Mirjana S.
Krstic, Aleksandra
Jovanović, Dragoljub
Raičević, Slavica
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 LT = x LT = 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to... more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright (C) 2010 Published by Elsevier B. V. All rights reserved.
Кључне речи:
Hydroxyapatite / Silver / Antimicrobial / Bone / Hemolysis / AFMИзвор:
Applied Surface Science, 2011, 257, 9, 4510-4518Финансирање / пројекти:
- Ministry of Science and Environmental Protection of the Republic of Serbia
DOI: 10.1016/j.apsusc.2010.12.113
ISSN: 0169-4332
WoS: 000286459600114
Scopus: 2-s2.0-78951487082
Колекције
Институција/група
VinčaTY - JOUR AU - Stanić, Vojislav AU - Janaćković, Đorđe T. AU - Dimitrijević, Suzana I. AU - Tanaskovic, Sladjana B. AU - Mitrić, Miodrag AU - Pavlović, Mirjana S. AU - Krstic, Aleksandra AU - Jovanović, Dragoljub AU - Raičević, Slavica PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4194 AB - Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 LT = x LT = 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright (C) 2010 Published by Elsevier B. V. All rights reserved. T2 - Applied Surface Science T1 - Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering VL - 257 IS - 9 SP - 4510 EP - 4518 DO - 10.1016/j.apsusc.2010.12.113 ER -
@article{ author = "Stanić, Vojislav and Janaćković, Đorđe T. and Dimitrijević, Suzana I. and Tanaskovic, Sladjana B. and Mitrić, Miodrag and Pavlović, Mirjana S. and Krstic, Aleksandra and Jovanović, Dragoljub and Raičević, Slavica", year = "2011", abstract = "Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 LT = x LT = 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright (C) 2010 Published by Elsevier B. V. All rights reserved.", journal = "Applied Surface Science", title = "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering", volume = "257", number = "9", pages = "4510-4518", doi = "10.1016/j.apsusc.2010.12.113" }
Stanić, V., Janaćković, Đ. T., Dimitrijević, S. I., Tanaskovic, S. B., Mitrić, M., Pavlović, M. S., Krstic, A., Jovanović, D.,& Raičević, S.. (2011). Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science, 257(9), 4510-4518. https://doi.org/10.1016/j.apsusc.2010.12.113
Stanić V, Janaćković ĐT, Dimitrijević SI, Tanaskovic SB, Mitrić M, Pavlović MS, Krstic A, Jovanović D, Raičević S. Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science. 2011;257(9):4510-4518. doi:10.1016/j.apsusc.2010.12.113 .
Stanić, Vojislav, Janaćković, Đorđe T., Dimitrijević, Suzana I., Tanaskovic, Sladjana B., Mitrić, Miodrag, Pavlović, Mirjana S., Krstic, Aleksandra, Jovanović, Dragoljub, Raičević, Slavica, "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering" in Applied Surface Science, 257, no. 9 (2011):4510-4518, https://doi.org/10.1016/j.apsusc.2010.12.113 . .