Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats
Abstract
Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidat...ive state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.
Keywords:
Stress / Liver / Antioxidant enzymes / Nuclear factor kappa B / Glucocorticoid receptorSource:
Physiological Research, 2010, 59, 5, 729-736Funding / projects:
- Odgovor sisarskih ćelija na endokrini i radiobiološki stres (RS-MESTD-MPN2006-2010-143042)
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Institution/Community
VinčaTY - JOUR AU - Đorđević, Jelena D. AU - Đorđević, Ana D. AU - Adžić, Miroslav AU - Nićiforović, Ana AU - Radojčić, Marija PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4153 AB - Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx. T2 - Physiological Research T1 - Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats VL - 59 IS - 5 SP - 729 EP - 736 UR - https://hdl.handle.net/21.15107/rcub_vinar_4153 ER -
@article{ author = "Đorđević, Jelena D. and Đorđević, Ana D. and Adžić, Miroslav and Nićiforović, Ana and Radojčić, Marija", year = "2010", abstract = "Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.", journal = "Physiological Research", title = "Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats", volume = "59", number = "5", pages = "729-736", url = "https://hdl.handle.net/21.15107/rcub_vinar_4153" }
Đorđević, J. D., Đorđević, A. D., Adžić, M., Nićiforović, A.,& Radojčić, M.. (2010). Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats. in Physiological Research, 59(5), 729-736. https://hdl.handle.net/21.15107/rcub_vinar_4153
Đorđević JD, Đorđević AD, Adžić M, Nićiforović A, Radojčić M. Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats. in Physiological Research. 2010;59(5):729-736. https://hdl.handle.net/21.15107/rcub_vinar_4153 .
Đorđević, Jelena D., Đorđević, Ana D., Adžić, Miroslav, Nićiforović, Ana, Radojčić, Marija, "Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats" in Physiological Research, 59, no. 5 (2010):729-736, https://hdl.handle.net/21.15107/rcub_vinar_4153 .