Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol
Drakulić, Dunja R.
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The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.
Keywords:mitochondria / estradiol / Ca2+ influx / Ca2+ efflux
Source:Archives of biological sciences, 2009, 61, 2, 171-177
- Serbian Ministry of Science