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dc.creatorKorićanac, Goran
dc.creatorMilosavljević, Tijana
dc.creatorStojiljković, Mojca D.
dc.creatorŽakula, Zorica
dc.creatorTepavčević, Snežana
dc.creatorRibarac-Stepić, Nevena B.
dc.creatorIsenović, Esma R.
dc.date.accessioned2018-03-01T20:43:04Z
dc.date.available2018-03-01T20:43:04Z
dc.date.issued2009
dc.identifier.issn0263-6484
dc.identifier.issn1099-0844
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/3648
dc.description.abstractIt is well known that variation in the concentration of estrogens affects insulin action. In this study we examine the impact of estradiol (E2) on insulin signaling in the rat heart. Ovariectomized female rats were treated with E2 6 h prior to analysis of basal protein and mRNA content of insulin signaling molecules, and additionally with insulin 30 min before the experiment to delineate E2 effects on phosphorylations and molecular associations relevant for insulin signaling. The results show that E2 decreased insulin receptor (IR) tyrosine phosphorylation, while it did not alter IR protein and mRNA content. E2 administration did not chance IR substrate 1 (IRS-1) protein content and tyrosine phosphorylation, while decreased mRNA content and increased its association with the p85 subunit of phosphatidylinositol 3-kinase (PI3K). E2 decreased protein and mRNA content of IR substrate 2 (IRS-2), while did not change IRS-2 tyrosine phosphorylation and IRS-2 association with p85. The increase of IRS-1/p85 is accompanied by increase of p85 protein and mRNA levels, and by stimulation of protein kinase B (Akt) Ser(473) phosphorylation. In contrast, Akt protein and mRNA content were not changed. In summary, although in some aspects cardiac insulin signaling is obviously improved by E2 treatment (increase of p85 mRNA and protein levels, enhancement of IRS-1/p85 association and Ser(473) Akt phosphorylation), the observed decrease of IR tyrosine phosphorylation, IRS-2 protein content, and IRSs mRNA contents, suggest very complex interplay of beneficial and suppressive effects of E2, both genomic and non-genomic, in regulation of heart insulin signaling. Copyright (C) 2009 John Wiley and Sons, Ltd.en
dc.relationMinistry of Science, Republic of Serbia [143030B]
dc.rightsrestrictedAccessen
dc.sourceCell Biochemistry and Functionen
dc.subject17 beta-estradiolen
dc.subjecthearten
dc.subjectinsulin signalingen
dc.subjectinsulin receptoren
dc.subjectPI3 kinaseen
dc.subjectAkten
dc.titleImpact of estradiol on insulin signaling in the rat hearten
dc.typearticleen
dcterms.abstractМилосављевиц, Тијана; Закула, Зорица; Рибарац-Степиц, Невена; Корићанац Горан; Стојиљковић Мојца; Тепавчевић Снежана; Исеновић Есма;
dc.citation.volume27
dc.citation.issue2
dc.citation.spage102
dc.citation.epage110
dc.identifier.wos000264118100007
dc.identifier.doi10.1002/cbf.1542
dc.citation.rankM23
dc.identifier.pmid19226537
dc.identifier.scopus2-s2.0-63149148931


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