The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells
Samo za registrovane korisnike
2008
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Prikaz svih podataka o dokumentuApstrakt
The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternat...ive proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.
Ključne reči:
prostate cancer / PC-3 cells / DU145 cells / cytotoxicity / ionizing radiation / 8-Cl-cAMP / apoptosisIzvor:
Investigational New Drugs, 2008, 26, 4, 309-317Finansiranje / projekti:
- Ministry of Science, Republic of Serbia [143009B]
- Ministry of Science, Republic of Serbia [143042B]
DOI: 10.1007/s10637-007-9101-4
ISSN: 0167-6997
PubMed: 18060599
WoS: 000256911400003
Scopus: 2-s2.0-45749135095
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Institucija/grupa
VinčaTY - JOUR AU - Vučić, Vesna AU - Nićiforović, Ana AU - Adžić, Miroslav AU - Radojčić, Marija AU - Ruždijić, Sabera PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3467 AB - The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer. T2 - Investigational New Drugs T1 - The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells VL - 26 IS - 4 SP - 309 EP - 317 DO - 10.1007/s10637-007-9101-4 ER -
@article{ author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Ruždijić, Sabera", year = "2008", abstract = "The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.", journal = "Investigational New Drugs", title = "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells", volume = "26", number = "4", pages = "309-317", doi = "10.1007/s10637-007-9101-4" }
Vučić, V., Nićiforović, A., Adžić, M., Radojčić, M.,& Ruždijić, S.. (2008). The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs, 26(4), 309-317. https://doi.org/10.1007/s10637-007-9101-4
Vučić V, Nićiforović A, Adžić M, Radojčić M, Ruždijić S. The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs. 2008;26(4):309-317. doi:10.1007/s10637-007-9101-4 .
Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Ruždijić, Sabera, "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells" in Investigational New Drugs, 26, no. 4 (2008):309-317, https://doi.org/10.1007/s10637-007-9101-4 . .