Modulation of tumor necrosis factor-mediated cell death by fullerenes
Нема приказа
Аутори
Harhaji, Ljubica M.
Isaković, Aleksandra J.
Vucicevic, Ljubica

Janjetović, Kristina D.

Misirkic, Maja
Marković, Zoran M.

Todorović-Marković, Biljana

Nikolić, Nadežda S.
Vranješ-Đurić, Sanja

Nikolić, Zoran M.

Trajković, Vladimir S.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Purpose. The fullerene (C-60/C-70 mixture-C-60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC(60/70)) and polyhydroxylated C-60/70 [C-60/70(OH)(n)] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNT). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC(60/70) augmented, while C-60/70(OH)(n) reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC(60/70) or C-60/70(OH)(n). The antioxidant N-acetylcysteine and mitochondrial per...meability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC(60/70) or C-60/70(OH)(n) potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage.
Кључне речи:
apoptosis / fullerene / necrosis / oxidative stress / tumor necrosis factorИзвор:
Pharmaceutical Research, 2008, 25, 6, 1365-1376
DOI: 10.1007/s11095-007-9486-y
ISSN: 0724-8741 (print)
PubMed: 17999162