Estrogen-induced modification of uterine RNA polymerase activity depends on localization of the estrogen receptor
2007
Аутори
Žakula, ZoricaIsenović, Esma R.
Stojiljković, Mojca D.
Tepavčević, Snežana
Ribarac-Stepić, Nevena B.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to examine the effects of estradiol (E2) on activity of RNA polymerase I and RNA polymerase II in uterine nuclei of ovariectomized (OVX) female rats. The obtained results show that estrogen-receptor (E-R) complexes in 30 min induced an increase of polymerase II activity. A second increase of polymerase II activity was observed after 3 h-incubation of nuclei with the E-R complex formed in the cytosol fraction. However, activity of polymerase I was increased 2 h after the start of incubation, with highest activity detected at 3 h in nuclei incubated with E-R complexes. On the contrary, no stimulatory effect on either polymerase I or polymerase II activity was observed in nuclei incubated with E2 alone. These results indicate that E2 stimulates the cytosolic estrogen receptor (ER), which in turn causes uterotrophic responses in OVX rats. In addition, they suggest that in order to provoke uterotrophic responses E-R complexes formed in the cytosol need to be retain...ed in the nucleus for a longer period of time.
Кључне речи:
estradiol / RNA polymerase I / RNA polymerase II / estrogen receptor / estradiol-receptor complex / uterusИзвор:
Archives of Biological Sciences, 2007, 59, 2, 105-112Финансирање / пројекти:
- Молекуларни механизми трансдукције хормонских сигнала: Биолошки маркери модификације и интеграције сигналних путева у физиолошким и патофизиолошким стањима (RS-MESTD-MPN2006-2010-143030)
Колекције
Институција/група
VinčaTY - JOUR AU - Žakula, Zorica AU - Isenović, Esma R. AU - Stojiljković, Mojca D. AU - Tepavčević, Snežana AU - Ribarac-Stepić, Nevena B. PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3412 AB - The aim of this study was to examine the effects of estradiol (E2) on activity of RNA polymerase I and RNA polymerase II in uterine nuclei of ovariectomized (OVX) female rats. The obtained results show that estrogen-receptor (E-R) complexes in 30 min induced an increase of polymerase II activity. A second increase of polymerase II activity was observed after 3 h-incubation of nuclei with the E-R complex formed in the cytosol fraction. However, activity of polymerase I was increased 2 h after the start of incubation, with highest activity detected at 3 h in nuclei incubated with E-R complexes. On the contrary, no stimulatory effect on either polymerase I or polymerase II activity was observed in nuclei incubated with E2 alone. These results indicate that E2 stimulates the cytosolic estrogen receptor (ER), which in turn causes uterotrophic responses in OVX rats. In addition, they suggest that in order to provoke uterotrophic responses E-R complexes formed in the cytosol need to be retained in the nucleus for a longer period of time. T2 - Archives of Biological Sciences T1 - Estrogen-induced modification of uterine RNA polymerase activity depends on localization of the estrogen receptor VL - 59 IS - 2 SP - 105 EP - 112 DO - 10.2298/ABS0702105Z ER -
@article{ author = "Žakula, Zorica and Isenović, Esma R. and Stojiljković, Mojca D. and Tepavčević, Snežana and Ribarac-Stepić, Nevena B.", year = "2007", abstract = "The aim of this study was to examine the effects of estradiol (E2) on activity of RNA polymerase I and RNA polymerase II in uterine nuclei of ovariectomized (OVX) female rats. The obtained results show that estrogen-receptor (E-R) complexes in 30 min induced an increase of polymerase II activity. A second increase of polymerase II activity was observed after 3 h-incubation of nuclei with the E-R complex formed in the cytosol fraction. However, activity of polymerase I was increased 2 h after the start of incubation, with highest activity detected at 3 h in nuclei incubated with E-R complexes. On the contrary, no stimulatory effect on either polymerase I or polymerase II activity was observed in nuclei incubated with E2 alone. These results indicate that E2 stimulates the cytosolic estrogen receptor (ER), which in turn causes uterotrophic responses in OVX rats. In addition, they suggest that in order to provoke uterotrophic responses E-R complexes formed in the cytosol need to be retained in the nucleus for a longer period of time.", journal = "Archives of Biological Sciences", title = "Estrogen-induced modification of uterine RNA polymerase activity depends on localization of the estrogen receptor", volume = "59", number = "2", pages = "105-112", doi = "10.2298/ABS0702105Z" }
Žakula, Z., Isenović, E. R., Stojiljković, M. D., Tepavčević, S.,& Ribarac-Stepić, N. B.. (2007). Estrogen-induced modification of uterine RNA polymerase activity depends on localization of the estrogen receptor. in Archives of Biological Sciences, 59(2), 105-112. https://doi.org/10.2298/ABS0702105Z
Žakula Z, Isenović ER, Stojiljković MD, Tepavčević S, Ribarac-Stepić NB. Estrogen-induced modification of uterine RNA polymerase activity depends on localization of the estrogen receptor. in Archives of Biological Sciences. 2007;59(2):105-112. doi:10.2298/ABS0702105Z .
Žakula, Zorica, Isenović, Esma R., Stojiljković, Mojca D., Tepavčević, Snežana, Ribarac-Stepić, Nevena B., "Estrogen-induced modification of uterine RNA polymerase activity depends on localization of the estrogen receptor" in Archives of Biological Sciences, 59, no. 2 (2007):105-112, https://doi.org/10.2298/ABS0702105Z . .