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dc.creatorBergman, Naomi
dc.creatorMoraes, Karen C. M.
dc.creatorAnderson, John R.
dc.creatorZarić, Božidarka
dc.creatorKambach, Christian
dc.creatorSchneider, Robert J.
dc.creatorWilusz, Carol J.
dc.creatorWilusz, Jeffrey
dc.date.accessioned2018-03-01T20:11:11Z
dc.date.available2018-03-01T20:11:11Z
dc.date.issued2007
dc.identifier.issn1545-9985
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/3277
dc.description.abstractMany orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 end. The precise function of this feature is unknown. Here we show that 5 poly( A) tracts are able to repress RNA decay by inhibiting 3 - to- 5 exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5 poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5 poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5 poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5 poly( A) tract. We propose that the Lsm complex simultaneously binds the 3 and 5 ends of these unusual messenger RNAs and thereby prevents 3 - to-5 decay. The implications of this phenomenon for cellular mRNA decay are discussed.en
dc.relationNIGMS NIH HHS [GM72481]
dc.rightsrestrictedAccessen
dc.sourceNature Structural and Molecular Biologyen
dc.titleLsm proteins bind and stabilize RNAs containing 5 poly(A) tractsen
dc.typearticleen
dcterms.abstractБергман, Наоми; Мораес, Карен Ц. М.; Aндерсон, Јохн Р.; Камбацх, Цхристиан; Wилусз, Царол Ј.; Wилусз, Јеффреy; Зарић Божидарка; Сцхнеидер, Роберт Ј.;
dc.citation.volume14
dc.citation.issue9
dc.citation.spage824
dc.citation.epage831
dc.identifier.wos000249276500009
dc.identifier.doi10.1038/nsmb1287
dc.citation.rankM21a
dc.identifier.pmid17694069
dc.identifier.scopus2-s2.0-34548472407


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