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Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage
dc.creator | Volarevic, Vladislav | |
dc.creator | Paunović, Verica G. | |
dc.creator | Marković, Zoran M. | |
dc.creator | Marković-Simović, Bojana | |
dc.creator | Misirkić-Marjanović, Maja | |
dc.creator | Todorović-Marković, Biljana | |
dc.creator | Bojic, Sanja | |
dc.creator | Vucicevic, Ljubica | |
dc.creator | Jovanović, Svetlana P. | |
dc.creator | Arsenijević, Nebojša N. | |
dc.creator | Holclajtner-Antunović, Ivanka D. | |
dc.creator | Milosavljević, Momir | |
dc.creator | Dramićanin, Miroslav | |
dc.creator | Kravić-Stevović, Tamara K. | |
dc.creator | Ćirić, Darko | |
dc.creator | Lukić, Miodrag L. | |
dc.creator | Trajković, Vladimir S. | |
dc.date.accessioned | 2018-03-01T15:46:50Z | |
dc.date.available | 2018-03-01T15:46:50Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1936-0851 | |
dc.identifier.issn | 1936-086X | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/324 | |
dc.description.abstract | We investigated the effect of large (40 nm) graphene quantum dots (GQDs) in concanavalin A (Con A; 12 mg/kg i.v.)-induced mouse hepatitis, a T cell-mediated liver injury resembling fulminant hepatitis in humans. Intravenously injected GQDs (50 mg/kg) accumulated in liver and reduced Con A-mediated liver damage, as demonstrated by histopathological analysis and a decrease in liver lipid peroxidation and serum levels of liver transaminases. The cleavage of apoptotic markers caspase-3/PARP and mRNA levels of proapoptotic mediators Puma, Noxa, Bax, Bak1, Bim, Apaf1, and p21, as well as LC3-I conversion to autophagosome-associated LC3-II and expression of autophagy-related (Atg) genes Atg4b, Atg7, Atg12, and beclin-1, were attenuated by GQDs, indicating a decrease in both apoptosis and autophagy in the liver tissue. This was associated with the reduced liver infiltration of immune cells, particularly the T cells producing proinflammatory cytokine IFN-?, and a decrease in IFN-gamma serum levels. In the spleen of GQD-exposed mice, mRNA expression of IFN-? and its transcription factor T-bet was reduced, while that of the IL-33 ligand ST2 was increased. The hepatoprotective effect of GQDs was less pronounced in ST2-deficient mice, indicating that it might depend on ST2 upregulation. In vitro, GQDs inhibited splenocyte IFN-gamma production, reduced the activation of extracellular signal-regulated kinase in macrophage and T cell lines, inhibited macrophage production of the free radical nitric oxide, and reduced its cytotoxicity toward hepatocyte cell line HepG2. Therefore, GQDs alleviate immune-mediated fulminant hepatitis by interfering with T cell and macrophage activation and possibly by exerting a direct hepatoprotective effect. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41025/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175069/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175103/RS// | |
dc.relation | Faculty of Medical Sciences University of Kragujevac, Serbia [MP01/12] | |
dc.rights | restrictedAccess | en |
dc.source | ACS Nano | en |
dc.subject | graphene | en |
dc.subject | quantum dot | en |
dc.subject | hepatitis | en |
dc.subject | apoptosis | en |
dc.subject | autophagy | en |
dc.title | Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage | en |
dc.type | article | en |
dcterms.abstract | Тодоровић-Марковић Биљана; Вуцицевиц, Љубица; Марковић Зоран; Aрсенијевиц, Небојса; Драмићанин Мирослав; Милосављевић Момир; Лукиц, Миодраг Л.; Холцлајтнер-Aнтуновиц, Иванка; Цириц, Дарко; Јовановиц, Светлана; Бојиц, Сања; Пауновиц, Верица; Воларевиц, Владислав; Мисиркиц-Марјановиц, Маја; Кравиц-Стевовиц, Тамара; Трајковиц, Владимир; Марковиц, Бојана Симовиц; | |
dc.citation.volume | 8 | |
dc.citation.issue | 12 | |
dc.citation.spage | 12098 | |
dc.citation.epage | 12109 | |
dc.identifier.wos | 000347138000023 | |
dc.identifier.doi | 10.1021/nn502466z | |
dc.citation.rank | M21a | |
dc.identifier.pmid | 25415137 | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-84919725926 |
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