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Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

dc.creatorŽivković, Maja
dc.creatorRakovic, Aleksandar
dc.creatorStanković, Aleksandra
dc.date.accessioned2018-03-01T19:51:25Z
dc.date.available2018-03-01T19:51:25Z
dc.date.issued2006
dc.identifier.issn0009-9120
dc.identifier.urihttps://vinar.vin.bg.ac.rs/handle/123456789/3045
dc.description.abstractObjectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening. (c) 2006 The Canadian, Society of Clinical Chemists. All rights reserved.en
dc.rightsrestrictedAccessen
dc.sourceClinical Biochemistryen
dc.subjectallele-specificen
dc.subjectgenotypingen
dc.subjectMMP-9en
dc.subjectpolymorphismen
dc.titleAllele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGEen
dc.typearticleen
dcterms.abstractРаковиц, Aлександар; Живковић Маја; Станковић Aлександра;
dc.citation.volume39
dc.citation.issue6
dc.citation.spage630
dc.citation.epage632
dc.identifier.wos000239195900011
dc.identifier.doi10.1016/j.clinbiochem.2006.01.005
dc.citation.rankM21
dc.identifier.pmid16458283
dc.identifier.scopus2-s2.0-33745477874


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