Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE
Чланак у часопису
МетаподациПриказ свих података о документу
Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening. (c) 2006 The Canadian, Society of Clinical Chemists. All rights reserved.
Кључне речи:allele-specific / genotyping / MMP-9 / polymorphism
Извор:Clinical Biochemistry, 2006, 39, 6, 630-632
ISSN: 0009-9120 (print)