Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE
Апстракт
Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening. (c) 2006 The Canadian, Society of Clinical Chemists. All rights reserved.
Кључне речи:
allele-specific / genotyping / MMP-9 / polymorphismИзвор:
Clinical Biochemistry, 2006, 39, 6, 630-632
DOI: 10.1016/j.clinbiochem.2006.01.005
ISSN: 0009-9120
PubMed: 16458283
WoS: 000239195900011
Scopus: 2-s2.0-33745477874
Колекције
Институција/група
VinčaTY - JOUR AU - Živković, Maja AU - Rakovic, Aleksandar AU - Stanković, Aleksandra PY - 2006 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3045 AB - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening. (c) 2006 The Canadian, Society of Clinical Chemists. All rights reserved. T2 - Clinical Biochemistry T1 - Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE VL - 39 IS - 6 SP - 630 EP - 632 DO - 10.1016/j.clinbiochem.2006.01.005 ER -
@article{ author = "Živković, Maja and Rakovic, Aleksandar and Stanković, Aleksandra", year = "2006", abstract = "Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening. (c) 2006 The Canadian, Society of Clinical Chemists. All rights reserved.", journal = "Clinical Biochemistry", title = "Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE", volume = "39", number = "6", pages = "630-632", doi = "10.1016/j.clinbiochem.2006.01.005" }
Živković, M., Rakovic, A.,& Stanković, A.. (2006). Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE. in Clinical Biochemistry, 39(6), 630-632. https://doi.org/10.1016/j.clinbiochem.2006.01.005
Živković M, Rakovic A, Stanković A. Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE. in Clinical Biochemistry. 2006;39(6):630-632. doi:10.1016/j.clinbiochem.2006.01.005 .
Živković, Maja, Rakovic, Aleksandar, Stanković, Aleksandra, "Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE" in Clinical Biochemistry, 39, no. 6 (2006):630-632, https://doi.org/10.1016/j.clinbiochem.2006.01.005 . .