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dc.creatorHorvat, Anica
dc.creatorMomić, Tatjana
dc.creatorBanjac, A.
dc.creatorPetrovic, S.
dc.creatorNikezic, G.
dc.creatorDemajo, M.
dc.date.accessioned2018-03-01T19:51:00Z
dc.date.available2018-03-01T19:51:00Z
dc.date.issued2006
dc.identifier.issn0862-8408 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/3040
dc.description.abstractThe effect of drugs from the class of cardiac (methyldigoxin, verapamil, propranolol), antiepileptic ( carbamazepine), sedative (diazepam) and antihistaminic (promethazine) drugs on Na,K-ATPase activity of plasma membranes was studied in rat brain synaptosomes. Methyldigoxin in a concentration of 0.1 mmol/l inhibits enzyme activity by 80%. Verapamil, propranolol and promethazine in concentrations of 20, 20 and 2 mmol/l respectively, entirely inhibit the ATPase activity. Carbamazepine and diazepam in concentrations of 0.02-60 mmol/l have no effect on the activity of this enzyme. According to the drug concentrations that inhibit 50% of enzyme activity (IC50), the potency can be listed in the following order: methyldigoxin GT GT promethazine GT verapamil GT = propranolol. From the inhibition of commercially available purified Na, K-ATPase isolated from porcine cerebral cortex in the presence of chosen drugs, as well as from kinetic studies on synaptosomal plasma membranes, it may be concluded that the drugs inhibit enzyme activity, partly by acting directly on the enzyme proteins. Propranolol, verapamil and promethazine inhibitions acted in an uncompetitive manner. The results suggest that these three drugs may contribute to neurological dysfunctions and indicate the necessity to take into consideration the side effects of the investigated drugs during the treatment of various pathological conditions.en
dc.rightsrestrictedAccessen
dc.sourcePhysiological Researchen
dc.subjectNa,K-ATPaseen
dc.subjectsynaptosomesen
dc.subjectverapamilen
dc.subjectpropranololen
dc.subjectpromethazineen
dc.titleSelective inhibition of brain Na,K-ATPase by drugsen
dc.typearticleen
dcterms.abstractБањац, A.; Петровиц, С.; Никезиц, Г.; Демајо, М.; Хорват, Aница; Момић Татјана;
dc.citation.volume55
dc.citation.issue3
dc.citation.spage325
dc.citation.epage338
dc.identifier.wos000238958200012
dc.citation.rankM22
dc.identifier.pmid16083303


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