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Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines
dc.creator | Tanić, Nikola | |
dc.creator | Brkić, G | |
dc.creator | Dimitrijević, Bogomir B. | |
dc.creator | Dedović-Tanić, Nasta | |
dc.creator | Gefen, N | |
dc.creator | Benharroch, D | |
dc.creator | Gopas, J | |
dc.date.accessioned | 2018-03-01T19:50:18Z | |
dc.date.available | 2018-03-01T19:50:18Z | |
dc.date.issued | 2006 | |
dc.identifier.issn | 0250-7005 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/3032 | |
dc.description.abstract | Background: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy. | en |
dc.rights | restrictedAccess | en |
dc.source | Anticancer Research | en |
dc.subject | melanoma | en |
dc.subject | drug resistance | en |
dc.subject | differentially expressed genes | en |
dc.subject | TCP 1-zeta-6A | en |
dc.subject | CD44 | en |
dc.subject | LPPR-2 | en |
dc.title | Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines | en |
dc.type | article | en |
dcterms.abstract | Таниц, Н; Бркиц, Г; Дедовиц-Таниц, Н; Гефен, Н; Бенхарроцх, Д; Димитријевић Богомир Б.; Гопас, Ј; | |
dc.citation.volume | 26 | |
dc.citation.issue | 3A | |
dc.citation.spage | 2137 | |
dc.citation.epage | 2142 | |
dc.identifier.wos | 000238490700057 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 16827156 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_vinar_3032 |
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