Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines
Nema prikaza
Autori
Tanić, NikolaBrkić, G
Dimitrijević, Bogomir B.
Dedović-Tanić, Nasta
Gefen, N
Benharroch, D
Gopas, J
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression ...were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy.
Ključne reči:
melanoma / drug resistance / differentially expressed genes / TCP 1-zeta-6A / CD44 / LPPR-2Izvor:
Anticancer Research, 2006, 26, 3A, 2137-2142Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Tanić, Nikola AU - Brkić, G AU - Dimitrijević, Bogomir B. AU - Dedović-Tanić, Nasta AU - Gefen, N AU - Benharroch, D AU - Gopas, J PY - 2006 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3032 AB - Background: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy. T2 - Anticancer Research T1 - Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines VL - 26 IS - 3A SP - 2137 EP - 2142 UR - https://hdl.handle.net/21.15107/rcub_vinar_3032 ER -
@article{ author = "Tanić, Nikola and Brkić, G and Dimitrijević, Bogomir B. and Dedović-Tanić, Nasta and Gefen, N and Benharroch, D and Gopas, J", year = "2006", abstract = "Background: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy.", journal = "Anticancer Research", title = "Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines", volume = "26", number = "3A", pages = "2137-2142", url = "https://hdl.handle.net/21.15107/rcub_vinar_3032" }
Tanić, N., Brkić, G., Dimitrijević, B. B., Dedović-Tanić, N., Gefen, N., Benharroch, D.,& Gopas, J.. (2006). Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines. in Anticancer Research, 26(3A), 2137-2142. https://hdl.handle.net/21.15107/rcub_vinar_3032
Tanić N, Brkić G, Dimitrijević BB, Dedović-Tanić N, Gefen N, Benharroch D, Gopas J. Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines. in Anticancer Research. 2006;26(3A):2137-2142. https://hdl.handle.net/21.15107/rcub_vinar_3032 .
Tanić, Nikola, Brkić, G, Dimitrijević, Bogomir B., Dedović-Tanić, Nasta, Gefen, N, Benharroch, D, Gopas, J, "Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines" in Anticancer Research, 26, no. 3A (2006):2137-2142, https://hdl.handle.net/21.15107/rcub_vinar_3032 .