Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats
Апстракт
Both the peripheral sympatho-adrenomedullary and central catecholaminergic systems are activated by various psycho-social and physical stressors. Catecholamine stores in the hypothalamus, hippocampus, adrenal glands, and heart auricles of long-term socially isolated (21 days) and control 3-month-old male Wistar rats, as well as their response to immobilization of all 4 limbs and head fixed for 2 h and cold stress (4 degrees C, 2 h), were studied. A simultaneous single isotope radioenzymatic assay based on the conversion of catecholamines to the corresponding O-methylated derivatives by catechol-O-methyltransferase in the presence of S-adenosyl-1-(H-3-methyl)-methionine was used. The O-methylated derivatives were oxidized to H-3-vanilline and the radioactivity measured. Social isolation produced depletion of hypothalamic norepinephrine (about 18%) and hippocampal dopamine (about 20%) stores and no changes in peripheral tissues. Immobilization decreased catecholamine stores (approximatel...y 39%) in central and peripheral tissues of control animals. However, in socially isolated rats, these reductions were observed only in the hippocampus and peripheral tissues. Cold did not affect hypothalamic catecholamine stores but reduced hippocampal. dopamine (about 20%) as well as norepinephrine stores in peripheral tissues both in control and socially isolated rats, while epinephrine levels were unchanged. Thus, immobilization was more efficient in reducing catecholamine stores in control and chronically isolated rats compared to cold stress. The differences in rearing conditions appear to influence the response of adult animals to additional stress. In addition, the influence of previous exposure to a stressor on catecholaminergic activity in the brainstem depends on both the particular catecholaminergic area studied and the properties of additional acute stress. Therefore, the sensitivity of the catecholaminergic system to habituation appears to be tissue-specific.
Кључне речи:
cold stress / immobilization stress / catecholamines / hypothalamus / hippocampus / adrenal glandsИзвор:
Brazilian Journal of Medical and Biological Research, 2006, 39, 6, 785-790
DOI: 10.1590/S0100-879X2006000600011
ISSN: 0100-879X
PubMed: 16751984
WoS: 000238305200011
Scopus: 2-s2.0-33745060760
Колекције
Институција/група
VinčaTY - JOUR AU - Dronjak, Slađana AU - Gavrilović, Ljubica PY - 2006 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3027 AB - Both the peripheral sympatho-adrenomedullary and central catecholaminergic systems are activated by various psycho-social and physical stressors. Catecholamine stores in the hypothalamus, hippocampus, adrenal glands, and heart auricles of long-term socially isolated (21 days) and control 3-month-old male Wistar rats, as well as their response to immobilization of all 4 limbs and head fixed for 2 h and cold stress (4 degrees C, 2 h), were studied. A simultaneous single isotope radioenzymatic assay based on the conversion of catecholamines to the corresponding O-methylated derivatives by catechol-O-methyltransferase in the presence of S-adenosyl-1-(H-3-methyl)-methionine was used. The O-methylated derivatives were oxidized to H-3-vanilline and the radioactivity measured. Social isolation produced depletion of hypothalamic norepinephrine (about 18%) and hippocampal dopamine (about 20%) stores and no changes in peripheral tissues. Immobilization decreased catecholamine stores (approximately 39%) in central and peripheral tissues of control animals. However, in socially isolated rats, these reductions were observed only in the hippocampus and peripheral tissues. Cold did not affect hypothalamic catecholamine stores but reduced hippocampal. dopamine (about 20%) as well as norepinephrine stores in peripheral tissues both in control and socially isolated rats, while epinephrine levels were unchanged. Thus, immobilization was more efficient in reducing catecholamine stores in control and chronically isolated rats compared to cold stress. The differences in rearing conditions appear to influence the response of adult animals to additional stress. In addition, the influence of previous exposure to a stressor on catecholaminergic activity in the brainstem depends on both the particular catecholaminergic area studied and the properties of additional acute stress. Therefore, the sensitivity of the catecholaminergic system to habituation appears to be tissue-specific. T2 - Brazilian Journal of Medical and Biological Research T1 - Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats VL - 39 IS - 6 SP - 785 EP - 790 DO - 10.1590/S0100-879X2006000600011 ER -
@article{ author = "Dronjak, Slađana and Gavrilović, Ljubica", year = "2006", abstract = "Both the peripheral sympatho-adrenomedullary and central catecholaminergic systems are activated by various psycho-social and physical stressors. Catecholamine stores in the hypothalamus, hippocampus, adrenal glands, and heart auricles of long-term socially isolated (21 days) and control 3-month-old male Wistar rats, as well as their response to immobilization of all 4 limbs and head fixed for 2 h and cold stress (4 degrees C, 2 h), were studied. A simultaneous single isotope radioenzymatic assay based on the conversion of catecholamines to the corresponding O-methylated derivatives by catechol-O-methyltransferase in the presence of S-adenosyl-1-(H-3-methyl)-methionine was used. The O-methylated derivatives were oxidized to H-3-vanilline and the radioactivity measured. Social isolation produced depletion of hypothalamic norepinephrine (about 18%) and hippocampal dopamine (about 20%) stores and no changes in peripheral tissues. Immobilization decreased catecholamine stores (approximately 39%) in central and peripheral tissues of control animals. However, in socially isolated rats, these reductions were observed only in the hippocampus and peripheral tissues. Cold did not affect hypothalamic catecholamine stores but reduced hippocampal. dopamine (about 20%) as well as norepinephrine stores in peripheral tissues both in control and socially isolated rats, while epinephrine levels were unchanged. Thus, immobilization was more efficient in reducing catecholamine stores in control and chronically isolated rats compared to cold stress. The differences in rearing conditions appear to influence the response of adult animals to additional stress. In addition, the influence of previous exposure to a stressor on catecholaminergic activity in the brainstem depends on both the particular catecholaminergic area studied and the properties of additional acute stress. Therefore, the sensitivity of the catecholaminergic system to habituation appears to be tissue-specific.", journal = "Brazilian Journal of Medical and Biological Research", title = "Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats", volume = "39", number = "6", pages = "785-790", doi = "10.1590/S0100-879X2006000600011" }
Dronjak, S.,& Gavrilović, L.. (2006). Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats. in Brazilian Journal of Medical and Biological Research, 39(6), 785-790. https://doi.org/10.1590/S0100-879X2006000600011
Dronjak S, Gavrilović L. Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats. in Brazilian Journal of Medical and Biological Research. 2006;39(6):785-790. doi:10.1590/S0100-879X2006000600011 .
Dronjak, Slađana, Gavrilović, Ljubica, "Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats" in Brazilian Journal of Medical and Biological Research, 39, no. 6 (2006):785-790, https://doi.org/10.1590/S0100-879X2006000600011 . .