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dc.creatorPetrović, Ivan M.
dc.creatorRistić-Fira, Aleksandra
dc.creatorTodorović, Danijela V.
dc.creatorValastro, L
dc.creatorCirrone, Giuseppe Antonio Pablo
dc.creatorCuttone, Giacomo
dc.date.accessioned2018-03-01T19:49:01Z
dc.date.available2018-03-01T19:49:01Z
dc.date.issued2006
dc.identifier.issn0955-3002 (print)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/123456789/3017
dc.description.abstractPurpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.en
dc.rightsclosedAccessen
dc.sourceInternational Journal of Radiation Biologyen
dc.subjectHTB140 melanoma cellsen
dc.subjectprotonsen
dc.subjectgamma-raysen
dc.subjectviabilityen
dc.subjectsurvivalen
dc.subjectapoptosisen
dc.subjectcell cycleen
dc.subjectanalysesen
dc.titleRadiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beamen
dc.typearticleen
dcterms.abstractПетровиц, И; Тодоровиц, Д; Ристић-Фира Aлександра; Валастро, Л; Цирроне, П; Цуттоне, Г;
dc.citation.volume82
dc.citation.issue4
dc.citation.spage251
dc.citation.epage265
dc.identifier.wos000237470700004
dc.identifier.doi10.1080/09553000600669859
dc.citation.rankM21a
dc.identifier.pmid16690593
dc.identifier.scopus2-s2.0-33646781232


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