Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam
Nema prikaza
Autori
Petrović, Ivan M.Ristić-Fira, Aleksandra
Todorović, Danijela V.
Valastro, Lucia M.
Cirrone, Giuseppe Antonio Pablo
Cuttone, Giacomo
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels w...ith time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.
Ključne reči:
HTB140 melanoma cells / protons / gamma-rays / viability / survival / apoptosis / cell cycle / analysesIzvor:
International Journal of Radiation Biology, 2006, 82, 4, 251-265
DOI: 10.1080/09553000600669859
ISSN: 0955-3002
PubMed: 16690593
WoS: 000237470700004
Scopus: 2-s2.0-33646781232
Institucija/grupa
VinčaTY - JOUR AU - Petrović, Ivan M. AU - Ristić-Fira, Aleksandra AU - Todorović, Danijela V. AU - Valastro, Lucia M. AU - Cirrone, Giuseppe Antonio Pablo AU - Cuttone, Giacomo PY - 2006 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3017 AB - Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays. T2 - International Journal of Radiation Biology T1 - Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam VL - 82 IS - 4 SP - 251 EP - 265 DO - 10.1080/09553000600669859 ER -
@article{ author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo", year = "2006", abstract = "Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.", journal = "International Journal of Radiation Biology", title = "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam", volume = "82", number = "4", pages = "251-265", doi = "10.1080/09553000600669859" }
Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P.,& Cuttone, G.. (2006). Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology, 82(4), 251-265. https://doi.org/10.1080/09553000600669859
Petrović IM, Ristić-Fira A, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G. Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology. 2006;82(4):251-265. doi:10.1080/09553000600669859 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam" in International Journal of Radiation Biology, 82, no. 4 (2006):251-265, https://doi.org/10.1080/09553000600669859 . .