Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats
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AuthorsObradović, Milan M.
Isenović, Esma R.
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We studied the in vivo effects of estradiol on size and biochemical parameters of cardiomyocytes in pathophysiological conditions such as obesity and insulin resistance. Male Wistar rats were normally fed (controls, n = 7) or fed with high-fat diet (obese, n = 14). Half of the obese rats (obese + estradiol, n = 7) were treated with a single dose of estradiol (40 g/kg, intraperitoneally) and 24 hours after treatment all the rats were killed. Estradiol in vivo in obese rats resulted in a significant increase in protein kinase B (Akt) activation (P LT .05) and decrease in heart mass (P LT .05), ratio of the heart mass/body mass (P LT .05), transverse diameters of cardiomyocytes (P LT .001), concentration of serum high-sensitivity C-reactive protein (P LT .001), and total cholesterol (P LT .01) compared with obese nontreated rats. Our results suggest that estradiol in obese/IR rats affects the size of cardiomyocytes and its actions lead in vivo to a reduction in obesity-induced cardiac hyp...ertrophy, via Akt.