Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays
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Ristić-Fira, AleksandraPetrović, Ivan M.
Todorović, Danijela V.
Korićanac, Lela
Vujčić, Miroslava T.
Demajo, Miroslav
Sabini, G
Cirrone, Giuseppe Antonio Pablo
Cuttone, Giacomo
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The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma ray...s, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).
Keywords:
HTB63 melanoma cells / protons / gamma rays / apoptosisSource:
Oncology Reports, 2004, 12, 6, 1323-1328Collections
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VinčaTY - JOUR AU - Ristić-Fira, Aleksandra AU - Petrović, Ivan M. AU - Todorović, Danijela V. AU - Korićanac, Lela AU - Vujčić, Miroslava T. AU - Demajo, Miroslav AU - Sabini, G AU - Cirrone, Giuseppe Antonio Pablo AU - Cuttone, Giacomo PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2829 AB - The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET). T2 - Oncology Reports T1 - Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays VL - 12 IS - 6 SP - 1323 EP - 1328 UR - https://hdl.handle.net/21.15107/rcub_vinar_2829 ER -
@article{ author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Danijela V. and Korićanac, Lela and Vujčić, Miroslava T. and Demajo, Miroslav and Sabini, G and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo", year = "2004", abstract = "The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).", journal = "Oncology Reports", title = "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays", volume = "12", number = "6", pages = "1323-1328", url = "https://hdl.handle.net/21.15107/rcub_vinar_2829" }
Ristić-Fira, A., Petrović, I. M., Todorović, D. V., Korićanac, L., Vujčić, M. T., Demajo, M., Sabini, G., Cirrone, G. A. P.,& Cuttone, G.. (2004). Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports, 12(6), 1323-1328. https://hdl.handle.net/21.15107/rcub_vinar_2829
Ristić-Fira A, Petrović IM, Todorović DV, Korićanac L, Vujčić MT, Demajo M, Sabini G, Cirrone GAP, Cuttone G. Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports. 2004;12(6):1323-1328. https://hdl.handle.net/21.15107/rcub_vinar_2829 .
Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Danijela V., Korićanac, Lela, Vujčić, Miroslava T., Demajo, Miroslav, Sabini, G, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays" in Oncology Reports, 12, no. 6 (2004):1323-1328, https://hdl.handle.net/21.15107/rcub_vinar_2829 .