ATPases as multi-response sensing system for various organic and inorganic analytes
Апстракт
The possibility of using synaptic plasma membrane (SPM) enzymes Na+/K+-ATPase and Mg2+-ATPase, isolated from rat brain, as a biological component of multi-response sensing system for detection of different compounds (alkaline and heavy metal salts, organic compounds) was studied. The method is based on the spectrophotometric determination of inorganic ortho-phosphate (P-i) that serves as a measure of the enzymatic activity in the presence of various analytes. The concentration of P-i, liberated by enzyme catalysed hydrolysis of adenosinetriphosphate (ATP), was followed spectrophotometrically, by single exposure to analytes or in the mixture. P-i was dose dependent on the analyte concentration. Alkaline elements (Na, K, Mg), heavy metals (Pb, Cd, Hg, Cu, Fe, Co, Zn), toxic organic compounds (pyridine, urea, chlorpyrifos), and some drugs (digoxin, gitoxin) showed diverse effects, inducing the inhibition or stimulation of the enzymes activity. Development of simple test method for simulta...neous detection of the investigated analytes based on the variation of medium assay composition was discussed.
Кључне речи:
ATPase / heavy metals / alkaline elements / organic compounds / detectionИзвор:
Monatshefte Fur Chemie, 2004, 135, 5, 605-614
DOI: 10.1007/s00706-003-0156-4
ISSN: 0026-9247
WoS: 000221154400015
Scopus: 2-s2.0-2442630251
Колекције
Институција/група
VinčaTY - JOUR AU - Vasić, Vesna M. AU - Krinulović, Katarina AU - Krstić, Danijela Z. AU - Momić, Tatjana AU - Horvat, Anica PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2765 AB - The possibility of using synaptic plasma membrane (SPM) enzymes Na+/K+-ATPase and Mg2+-ATPase, isolated from rat brain, as a biological component of multi-response sensing system for detection of different compounds (alkaline and heavy metal salts, organic compounds) was studied. The method is based on the spectrophotometric determination of inorganic ortho-phosphate (P-i) that serves as a measure of the enzymatic activity in the presence of various analytes. The concentration of P-i, liberated by enzyme catalysed hydrolysis of adenosinetriphosphate (ATP), was followed spectrophotometrically, by single exposure to analytes or in the mixture. P-i was dose dependent on the analyte concentration. Alkaline elements (Na, K, Mg), heavy metals (Pb, Cd, Hg, Cu, Fe, Co, Zn), toxic organic compounds (pyridine, urea, chlorpyrifos), and some drugs (digoxin, gitoxin) showed diverse effects, inducing the inhibition or stimulation of the enzymes activity. Development of simple test method for simultaneous detection of the investigated analytes based on the variation of medium assay composition was discussed. T2 - Monatshefte Fur Chemie T1 - ATPases as multi-response sensing system for various organic and inorganic analytes VL - 135 IS - 5 SP - 605 EP - 614 DO - 10.1007/s00706-003-0156-4 ER -
@article{ author = "Vasić, Vesna M. and Krinulović, Katarina and Krstić, Danijela Z. and Momić, Tatjana and Horvat, Anica", year = "2004", abstract = "The possibility of using synaptic plasma membrane (SPM) enzymes Na+/K+-ATPase and Mg2+-ATPase, isolated from rat brain, as a biological component of multi-response sensing system for detection of different compounds (alkaline and heavy metal salts, organic compounds) was studied. The method is based on the spectrophotometric determination of inorganic ortho-phosphate (P-i) that serves as a measure of the enzymatic activity in the presence of various analytes. The concentration of P-i, liberated by enzyme catalysed hydrolysis of adenosinetriphosphate (ATP), was followed spectrophotometrically, by single exposure to analytes or in the mixture. P-i was dose dependent on the analyte concentration. Alkaline elements (Na, K, Mg), heavy metals (Pb, Cd, Hg, Cu, Fe, Co, Zn), toxic organic compounds (pyridine, urea, chlorpyrifos), and some drugs (digoxin, gitoxin) showed diverse effects, inducing the inhibition or stimulation of the enzymes activity. Development of simple test method for simultaneous detection of the investigated analytes based on the variation of medium assay composition was discussed.", journal = "Monatshefte Fur Chemie", title = "ATPases as multi-response sensing system for various organic and inorganic analytes", volume = "135", number = "5", pages = "605-614", doi = "10.1007/s00706-003-0156-4" }
Vasić, V. M., Krinulović, K., Krstić, D. Z., Momić, T.,& Horvat, A.. (2004). ATPases as multi-response sensing system for various organic and inorganic analytes. in Monatshefte Fur Chemie, 135(5), 605-614. https://doi.org/10.1007/s00706-003-0156-4
Vasić VM, Krinulović K, Krstić DZ, Momić T, Horvat A. ATPases as multi-response sensing system for various organic and inorganic analytes. in Monatshefte Fur Chemie. 2004;135(5):605-614. doi:10.1007/s00706-003-0156-4 .
Vasić, Vesna M., Krinulović, Katarina, Krstić, Danijela Z., Momić, Tatjana, Horvat, Anica, "ATPases as multi-response sensing system for various organic and inorganic analytes" in Monatshefte Fur Chemie, 135, no. 5 (2004):605-614, https://doi.org/10.1007/s00706-003-0156-4 . .