In vivo monitoring of chlorophyll fluorescence response to low-dose gamma-irradiation in pumpkin (Cucurbita pepo) leaves
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The effect of gamma-irradiation on the in vivo-measured chlorophyll fluorescence in a pumpkin leaves (Cucurbita pepo) has been investigated. Plants were grown in the same environment, then divided into several groups and irradiated at ambient conditions at small dose levels (up to 13.4 Gy) with Co-60 gamma-rays. The post-irradiation effect on chlorophyll status in the leaves was examined by measuring chlorophyll fluorescence 2 days (48 h) after exposure. It is undoubtedly found that the value of fluorescence intensity ratio (FIR) at 690 nm and 735 nm (F-690/F-735) depends upon the ionizing radiation dose. Even with the smallest dose of 3.35 Gy, ionizing radiation notably altered the fluorescence spectra of leaves. The spectra difference was manifested by decrease of FIR due to changed chlorophyll luminescence, the possible reason for which could be increase of chlorophyll concentration during the recovery process of the plant. The potential implications of these results for plant physi...ological status monitoring, as well as for pollution detection and assessment, are discussed in brief. Copyright (C) 2003 John Wiley Sons, Ltd.
Кључне речи:chlorophyll fluorescence / low-dose radiation
Извор:Luminescence, 2003, 18, 5, 274-277
ISSN: 1522-7235 (print)