The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain
Апстракт
The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 mug EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15. and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB on CuZnSOD activity in newborn females (51.8%) changed to an inhibitory effect on day 8 (38.4%) and disappeared by day 45 when inhibition was detected again (48.7%). In males, the inhibitory effect on this enzyme was observed on da...ys 0 (45.0%) and 15 (28.9%), and then disappeared until day 60 when a stimulatory effect was observed (38.4%). EB treatment had no effect on the other age groups. The sensitivity of MnSOD to estradiol differed significantly between sexes during the neonatal and prepubertal period, whereas it followed a similar pattern thereafter. The sensitivity of CuZnSOD to estradiol differed significantly between sexes during most of the study period. Regression analysis showed that the sensitivity of MnSOD to this estrogen tended to decrease similarly in both sexes, whereas the sensitivity of CuZnSOD showed a significantly different opposite tendency in female and male rats. These are the first reports indicating hormonal modulation of antioxidant enzyme activities related to the developmental process.
Кључне речи:
brain / brain development / estradiol / superoxide dismutaseИзвор:
Brazilian Journal of Medical and Biological Research, 2003, 36, 5, 579-586
DOI: 10.1590/S0100-879X2003000500004
ISSN: 0100-879X
PubMed: 12715076
WoS: 000183100000004
Scopus: 2-s2.0-0038279744
Колекције
Институција/група
VinčaTY - JOUR AU - Pejić, Snežana AU - Kasapović, Jelena AU - Cvetković, D AU - Pajović, Snežana B. PY - 2003 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2634 AB - The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 mug EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15. and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB on CuZnSOD activity in newborn females (51.8%) changed to an inhibitory effect on day 8 (38.4%) and disappeared by day 45 when inhibition was detected again (48.7%). In males, the inhibitory effect on this enzyme was observed on days 0 (45.0%) and 15 (28.9%), and then disappeared until day 60 when a stimulatory effect was observed (38.4%). EB treatment had no effect on the other age groups. The sensitivity of MnSOD to estradiol differed significantly between sexes during the neonatal and prepubertal period, whereas it followed a similar pattern thereafter. The sensitivity of CuZnSOD to estradiol differed significantly between sexes during most of the study period. Regression analysis showed that the sensitivity of MnSOD to this estrogen tended to decrease similarly in both sexes, whereas the sensitivity of CuZnSOD showed a significantly different opposite tendency in female and male rats. These are the first reports indicating hormonal modulation of antioxidant enzyme activities related to the developmental process. T2 - Brazilian Journal of Medical and Biological Research T1 - The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain VL - 36 IS - 5 SP - 579 EP - 586 DO - 10.1590/S0100-879X2003000500004 ER -
@article{ author = "Pejić, Snežana and Kasapović, Jelena and Cvetković, D and Pajović, Snežana B.", year = "2003", abstract = "The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 mug EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15. and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB on CuZnSOD activity in newborn females (51.8%) changed to an inhibitory effect on day 8 (38.4%) and disappeared by day 45 when inhibition was detected again (48.7%). In males, the inhibitory effect on this enzyme was observed on days 0 (45.0%) and 15 (28.9%), and then disappeared until day 60 when a stimulatory effect was observed (38.4%). EB treatment had no effect on the other age groups. The sensitivity of MnSOD to estradiol differed significantly between sexes during the neonatal and prepubertal period, whereas it followed a similar pattern thereafter. The sensitivity of CuZnSOD to estradiol differed significantly between sexes during most of the study period. Regression analysis showed that the sensitivity of MnSOD to this estrogen tended to decrease similarly in both sexes, whereas the sensitivity of CuZnSOD showed a significantly different opposite tendency in female and male rats. These are the first reports indicating hormonal modulation of antioxidant enzyme activities related to the developmental process.", journal = "Brazilian Journal of Medical and Biological Research", title = "The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain", volume = "36", number = "5", pages = "579-586", doi = "10.1590/S0100-879X2003000500004" }
Pejić, S., Kasapović, J., Cvetković, D.,& Pajović, S. B.. (2003). The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain. in Brazilian Journal of Medical and Biological Research, 36(5), 579-586. https://doi.org/10.1590/S0100-879X2003000500004
Pejić S, Kasapović J, Cvetković D, Pajović SB. The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain. in Brazilian Journal of Medical and Biological Research. 2003;36(5):579-586. doi:10.1590/S0100-879X2003000500004 .
Pejić, Snežana, Kasapović, Jelena, Cvetković, D, Pajović, Snežana B., "The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain" in Brazilian Journal of Medical and Biological Research, 36, no. 5 (2003):579-586, https://doi.org/10.1590/S0100-879X2003000500004 . .