Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes
Abstract
Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.
Keywords:
Na,K-ATPase / synaptic plasma membranes / immobilization / inhibition / cadmium / mercurySource:
Journal of the Serbian Chemical Society, 2002, 67, 12, 809-817
DOI: 10.2298/JSC0212809M
ISSN: 0352-5139
WoS: 000180192500003
Scopus: 2-s2.0-0036981182
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VinčaTY - JOUR AU - Momić, Tatjana AU - Vujčić, Zoran AU - Vasić, Vesna M. AU - Horvat, Anica PY - 2002 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2597 AB - Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples. T2 - Journal of the Serbian Chemical Society T1 - Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes VL - 67 IS - 12 SP - 809 EP - 817 DO - 10.2298/JSC0212809M ER -
@article{ author = "Momić, Tatjana and Vujčić, Zoran and Vasić, Vesna M. and Horvat, Anica", year = "2002", abstract = "Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.", journal = "Journal of the Serbian Chemical Society", title = "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes", volume = "67", number = "12", pages = "809-817", doi = "10.2298/JSC0212809M" }
Momić, T., Vujčić, Z., Vasić, V. M.,& Horvat, A.. (2002). Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society, 67(12), 809-817. https://doi.org/10.2298/JSC0212809M
Momić T, Vujčić Z, Vasić VM, Horvat A. Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society. 2002;67(12):809-817. doi:10.2298/JSC0212809M .
Momić, Tatjana, Vujčić, Zoran, Vasić, Vesna M., Horvat, Anica, "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes" in Journal of the Serbian Chemical Society, 67, no. 12 (2002):809-817, https://doi.org/10.2298/JSC0212809M . .