Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy
Апстракт
We studied the effect of total body X-irradiation and partial hepatectomy on the acute phase protein gene expression in rat liver. Male rats of AO strain were irradiated with high X-ray doses, without any visible tissue damage. In contrast, partial hepatectomy consisted of surgical removal of 40% liver tissue. The changes in liver mRNA concentrations for positive acute-phase reactants including cysteine protease inhibitor, alpha(1)-acid glycoprotein, fibrinogen and haptoglobin, and albumin as a negative reactant were monitored by Northern blot and slot-blot hybridizations using corresponding [P-32]dCTP labeled cDNA probes. While in the first 24 h after the partial hepatectomy, liver mRNA levels for the positive acute-phase reactants increased, briefly followed by an immediate decrease, the duration and timing of the acute-phase responses to the whole body X-irradiation were slightly different and lasted for as long as 72 h. Although both treatments induced the mRNA expression of acute-...phase reactants in rat liver, the observed variations in the duration and intensity of the changes in mRNA levels for the acute-phase proteins in these two types of tissue damage suggest the involvement of specific mechanisms in a fine tuning of the non-specific acute-phase responses to meet the unique requirements of the particular injury. (C) 2002 Elsevier Science Inc. All rights reserved.
Кључне речи:
acute-phase response / alpha l-acid glycoprotein (AGP) / albumin (Al) / cysteine protease inhibitor (CPI) / fibrinogen (Fb) / haptoglobin (Hp) / mRNA level / partial hepatectomy / rat liver / X-irradiationИзвор:
Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology, 2002, 133, 3, 461-470
DOI: 10.1016/S1532-0456(02)00174-6
ISSN: 1532-0456
PubMed: 12379430
WoS: 000179005500012
Scopus: 2-s2.0-0036837132
Колекције
Институција/група
VinčaTY - JOUR AU - Trutic, N AU - Magic, Z AU - Urosevic, N AU - Krtolica-Žikić, Koviljka PY - 2002 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2577 AB - We studied the effect of total body X-irradiation and partial hepatectomy on the acute phase protein gene expression in rat liver. Male rats of AO strain were irradiated with high X-ray doses, without any visible tissue damage. In contrast, partial hepatectomy consisted of surgical removal of 40% liver tissue. The changes in liver mRNA concentrations for positive acute-phase reactants including cysteine protease inhibitor, alpha(1)-acid glycoprotein, fibrinogen and haptoglobin, and albumin as a negative reactant were monitored by Northern blot and slot-blot hybridizations using corresponding [P-32]dCTP labeled cDNA probes. While in the first 24 h after the partial hepatectomy, liver mRNA levels for the positive acute-phase reactants increased, briefly followed by an immediate decrease, the duration and timing of the acute-phase responses to the whole body X-irradiation were slightly different and lasted for as long as 72 h. Although both treatments induced the mRNA expression of acute-phase reactants in rat liver, the observed variations in the duration and intensity of the changes in mRNA levels for the acute-phase proteins in these two types of tissue damage suggest the involvement of specific mechanisms in a fine tuning of the non-specific acute-phase responses to meet the unique requirements of the particular injury. (C) 2002 Elsevier Science Inc. All rights reserved. T2 - Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology T1 - Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy VL - 133 IS - 3 SP - 461 EP - 470 DO - 10.1016/S1532-0456(02)00174-6 ER -
@article{ author = "Trutic, N and Magic, Z and Urosevic, N and Krtolica-Žikić, Koviljka", year = "2002", abstract = "We studied the effect of total body X-irradiation and partial hepatectomy on the acute phase protein gene expression in rat liver. Male rats of AO strain were irradiated with high X-ray doses, without any visible tissue damage. In contrast, partial hepatectomy consisted of surgical removal of 40% liver tissue. The changes in liver mRNA concentrations for positive acute-phase reactants including cysteine protease inhibitor, alpha(1)-acid glycoprotein, fibrinogen and haptoglobin, and albumin as a negative reactant were monitored by Northern blot and slot-blot hybridizations using corresponding [P-32]dCTP labeled cDNA probes. While in the first 24 h after the partial hepatectomy, liver mRNA levels for the positive acute-phase reactants increased, briefly followed by an immediate decrease, the duration and timing of the acute-phase responses to the whole body X-irradiation were slightly different and lasted for as long as 72 h. Although both treatments induced the mRNA expression of acute-phase reactants in rat liver, the observed variations in the duration and intensity of the changes in mRNA levels for the acute-phase proteins in these two types of tissue damage suggest the involvement of specific mechanisms in a fine tuning of the non-specific acute-phase responses to meet the unique requirements of the particular injury. (C) 2002 Elsevier Science Inc. All rights reserved.", journal = "Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology", title = "Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy", volume = "133", number = "3", pages = "461-470", doi = "10.1016/S1532-0456(02)00174-6" }
Trutic, N., Magic, Z., Urosevic, N.,& Krtolica-Žikić, K.. (2002). Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy. in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology, 133(3), 461-470. https://doi.org/10.1016/S1532-0456(02)00174-6
Trutic N, Magic Z, Urosevic N, Krtolica-Žikić K. Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy. in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology. 2002;133(3):461-470. doi:10.1016/S1532-0456(02)00174-6 .
Trutic, N, Magic, Z, Urosevic, N, Krtolica-Žikić, Koviljka, "Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy" in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology, 133, no. 3 (2002):461-470, https://doi.org/10.1016/S1532-0456(02)00174-6 . .